Rapid and marker-free gene replacement in citric acid-producing Aspergillus tubingensis (A. niger) WU-2223L by the CRISPR/Cas9 system-based genome editing technique using DNA fragments encoding sgRNAs
Isato Yoshioka, Kohtaro Kirimura

Deletion of Aspergillus nidulans cpsA/rseA induces increased extracellular hydrolase production in solid-state culture partly through the high osmolarity glycerol pathway
Masahiro Ogawa, Hiroki Wada, Taro Yoshimura, Atsushi Sato, ... Hiroyuki Horiuchi

Asymmetric synthesis of intermediate for (1R,2S)-ethyl 1-amino-2-vinylcyclopropanecarboxylate by desymmetrization using engineered esterase from Bacillus subtilis
Hiroshi Kawabata, Ryoma Miyake, Kuniko Asada, Yasumasa Dekishima, ... Ryohei Kato

Structures of two novel crystal forms of Aspergillus oryzae alpha amylase (taka-amylase)
Christine L. Gee, James M. Holton, Alexander McPherson

Isolation and characterization of Lipomyces starkeyi mutants with greatly increased lipid productivity following UV irradiation
Hiroaki Takaku, Sayaka Ebina, Kotoha Kasuga, Rikako Sato, ... Harutake Yamazaki

High cellulolytic potential of the Ktedonobacteria lineage revealed by genome-wide analysis of CAZymes
Yu Zheng, Mayumi Maruoka, Kei Nanatani, Masafumi Hidaka, ... Shuhei Yabe

Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids
Chih-Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, ... Kazuhito Fujiyama

Extra copy of the mitochondrial cytochrome-c peroxidase gene confers a pyruvate-underproducing characteristic of sake yeast through respiratory metabolism
Yuki Fujimaru, Yuki Kusaba, Nairui Zhang, Huanghuang Dai, ... Hiroshi Kitagaki

Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate
Kaustubh Chandrakant Khaire, Kedar Sharma, Abhijeet Thakur, Vijayanand Suryakant Moholkar, Arun Goyal

Effect of honeycomb, granular, and powder activated carbon additives on continuous lactic acid fermentation of complex food waste with mixed inoculation
Qiao Wang, Huan Li, Kai Feng

Immobilization of nitrifying bacteria on composite based on polymers and eggshells for nitrate production
Mona Chaali, Hugo Alejandro Rivera Ortiz, Beatriz Delgado Cano, Satinder Kaur Brar, ... Michèle Heitz

Exosomes from adipose derived mesenchymal stem cells alleviate diabetic osteoporosis in rats through suppressing NLRP3 inflammasome activation in osteoclasts
Lei Zhang, Qinghai Wang, Hang Su, Jiaxiang Cheng

Impact of adipose-derived stem cells on engineering hair follicle germ-like tissue grafts for hair regenerative medicine
Rikuma Nakajima, Yoshiki Tate, Lei Yan, Tatsuto Kageyama, Junji Fukuda

Exogenous FGF-2 prolongs endothelial connection in multilayered human skeletal muscle cell sheet
Parichut Thummarati, Masahiro Kino-oka

Rapid and reliable hybridoma screening method that is suitable for production of functional structure-recognizing monoclonal antibody
Atsumi Sakaguchi, Chika Nakajima, Ayuko Sawano, Yoichiro Tanaka, Yasuyuki Kurihara