Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell
Xiang-Hui Qi, Jing-Fei Zhu, Jun-Hua Yun, Jing Lin, Yi-Lin Qi, Qi Guo, Hong Xu

Highlights

・The properties and kinetic parameter of xylitol dehydrogenase were determined.
・Xylitol dehydrogenase has a NAD(H)-binding motif and a YXXXK active site motif.
・Xylitol production was improved by mixed culture with adding exogenous NADH.
・The highest yield and volumetric productivity of xylitol were reported.

Inhibitory effect of Cinnamomum osmophloeum Kanehira ethanol extracts on melanin synthesis via repression of tyrosinase expression
Shih-Chieh Lee, Chun-Hao Chen, Chih-Wen Yu, Hsiao Ling Chen, Wei-Tung Huang, Yun-Shiang Chang, Shu-Hsien Hung, Tai-Lin Lee
Enzymology, Protein Engineering, and Enzyme Technology

Improvement of enantioselectivity of the B-type halohydrin hydrogen-halide-lyase from Corynebacterium sp. N-1074
Fumiaki Watanabe, Fujio Yu, Akashi Ohtaki, Yasuaki Yamanaka, Keiichi Noguchi, Masafumi Odaka, Masafumi Yohda

Salt-induced increase in the yield of enzymatically synthesized phosphatidylinositol and the underlying mechanism
Michiko Muraki, Jasmina Damnjanovic, Hideo Nakano, Yugo Iwasaki

Alleviation of temperature-sensitive secretion defect of Pseudomonas fluorescens ATP-binding cassette (ABC) transporter, TliDEF, by a change of single amino acid in the ABC protein, TliD
Gyeong Tae Eom, Joon Young Oh, Ji Hyun Park, Hye Jin Lim, So Jeong Lee, Eun Young Kim, Ji-Eun Choi, Jonggeon Jegal, Bong Keun Song, Ju-Hyun Yu, Jae Kwang Song

Screening, expression, and characterization of an anti-human oxidized low-density lipoprotein single-chain variable fragment
Miyuki Kumano-Kuramochi, Takashi Fujimura, Shiro Komba, Mari Maeda-Yamamoto, Sachiko Machida

Improvement of lipid content of Chlorella minutissima MCC 5 for biodiesel production
Sourabh Chakraborty, Debabrata Mohanty, Supratim Ghosh, Debabrata Das

Highlights

・Maximization of biomass production by single parameter optimization.
・Relative effectiveness of each parameter towards biomass production using Taguchi model.
・Determination of activation energy of Chlorella minutissima MCC 5.
・Enhancement of lipid content of C. minutissima MCC 5 under nitrate and phosphate limited condition・Suitability of C. minutissima MCC 5 as a feedstock for biodiesel production.

Recovery of laccase from processed Hericium erinaceus (Bull.:Fr) Pers. fruiting bodies in aqueous two-phase system
Devamalini Rajagopalu, Pau Loke Show, Yee Shin Tan, Sekaran Muniandy, Vikineswary Sabaratnam, Tau Chuan Ling

Highlights

・Laccase was successful recovered from processed H. erinaceus in PEG/Salt ATPS.
・Cold-stored of H. erinaceus recorded highest laccase activities (2.02 ± 0.04 U/mL).
・Recovery conditions of laccase in optimized system of APTS contained 17% (w/w) PEG-8000 and 12.2% (w/w) potassium phosphate.

Denitrification by Pseudomonas stutzeri coupled with CO2 reduction by Sporomusa ovata with hydrogen as an electron donor assisted by solid-phase humin
Zhixing Xiao, Takanori Awata, Dongdong Zhang, Arata Katayama

Highlights

・Denitrifying co-culture of Pseudomonas and Sporomusa was established.
・Denitrification was coupled with acetogenesis from CO2 with H2.
・Humin increased nitrate reducing capacity of co-culture when acetate was deficient.
・More than 60% of consumed H2 in co-culture system was used for denitrification.
・The co-culture system showed robust denitrification by successive transfer.

Comparison of amino and epoxy functionalized SBA-15 used for carbonic anhydrase immobilization
Xiaoyao Fei, Shaoyun Chen, Dai Liu, Chunjie Huang, Yongchun Zhang

Comparison of electrochemical and microbiological characterization of microbial fuel cells equipped with SPEEK and Nafion membrane electrode assemblies
Kei Suzuki, Rubaba Owen, Joann Mok, Hiroki Mochihara, Takuya Hosokawa, Hiroko Kubota, Hisatoshi Sakamoto, Atsunori Matsuda, Yosuke Tashiro, Hiroyuki Futamata

Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Masaki Torimura

Highlights

・We exposed mESCs to p-dichlorobenzene 1 or 28 days (daily dose).
・Mechanisms modulating proteins occurred following acute and chronic exposures.
・LncRNAs are novel biomarkers of the chemical stress response in mESCs.

Variation of the microbial community in thermophilic anaerobic digestion of pig manure mixed with different ratios of rice straw
Sheng Zhou, Marcell Nikolausz, Jining Zhang, Shohei Riya, Akihiko Terada, Masaaki Hosomi

Kinetics of bisphenol A degradation by Sphingomonas paucimobilis FJ-4
Hitoshi Fujiwara, Satoshi Soda, Masanori Fujita, Michihiko Ike

Responses of soil microbial biomass and bacterial community structure to closed-off management (an ecological natural restoration measures): A case study of Dongting Lake wetland, middle China
Juan Dai, Haipeng Wu, Chang Zhang, Guangming Zeng, Jie Liang, Shenglian Guo, Xiaodong Li, Lu Huang, Lunhui Lu, Yujie Yuan

Highlights

・Closed-off management applied to restore and protect many important wetlands.
・We compared soil microbial indicators in management area (MA) and contrast area (CA).
・Soil microbial biomass and diversity had significant increases after management.
・Functional marker genes of nitrogen cycling also had significant changes.

A simple agroinfiltration method for transient gene expression in plant leaf discs
Kouki Matsuo, Noriho Fukuzawa, Takeshi Matsumura

Differentiation signalobody: Demonstration of antigen-dependent osteoclast differentiation from a progenitor cell line
Hideto Nakabayashi, Saeko Aoyama, Masahiro Kawahara, Teruyuki Nagamune

Calorimetric studies of the growth of anaerobic microbes
Hideo Miyake, Yukiko Maeda, Takashi Ishikawa, Akiyoshi Tanaka

In vitro steroid profiling system for the evaluation of endocrine disruptors
Yosuke Nakano, Toshiyuki Yamashita, Masashi Okuno, Eiichiro Fukusaki, Takeshi Bamba