TORC1 activity is partially reduced under nitrogen starvation conditions in sake yeast Kyokai no. 7, Saccharomyces cerevisiae
Nobushige Nakazawa, Aya Sato, Masahiro Hosaka

Biochemical characterization of a bifunctional acetaldehyde-alcohol dehydrogenase purified from a facultative anaerobic bacterium Citrobacter sp. S-77
Kohsei Tsuji, Ki-Seok Yoon, Seiji Ogo

Purification and enzymatic characterization of a novel β-1,6-glucosidase from Aspergillus oryzae
Akira Watanabe, Moe Suzuki, Seiryu Ujiie, Katsuya Gomi

Improved conversion of cinnamaldehyde derivatives to diol compounds via a pyruvate decarboxylase-dependent mechanism in budding yeast
Shunichi Miyakoshi, Yukari Negishi, Yusuke Sekiya, Satoshi Nakajima

Glycerol acts as alternative electron sink during syngas fermentation by thermophilic anaerobe Moorella thermoacetica
Zen-ichiro Kimura, Akihisa Kita, Yuki Iwasaki, Yutaka Nakashimada, Tamotsu Hoshino, Katsuji Murakami

Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry reveals the correlation between chemical compounds in Japanese sake and its organoleptic properties
Kei Takahashi, Fumie Kabashima, Fumihiko Tsuchiya

Formation of ethyl ferulate from feruloylated oligosaccharide by transesterification of rice koji enzyme under sake mash conditions
Nobukazu Suzuki, Toshihiko Ito, Kai Hiroshima, Tetsuo Tokiwano, Katsumi Hashizume

Bacterial community structure and predicted alginate metabolic pathway in an alginate-degrading bacterial consortium
Akihisa Kita, Toyokazu Miura, Satoshi Kawata, Takeshi Yamaguchi, Yoshiko Okamura, Tsunehiro Aki, Yukihiko Matsumura, Takahisa Tajima, Junichi Kato, Naomichi Nishio, Yutaka Nakashimada

Highlights

・Alginate-degrading bacterial consortium was obtained from sand on the beach.
・This consortium was mainly composed of two dominant genera.
・This consortium possessed all the candidate genes involved in the alginate metabolic pathway.

Enhanced volatile fatty acids production of waste activated sludge under salinity conditions: Performance and mechanisms
Gaoqiang Su, Shuying Wang, Zhiguo Yuan, Yongzhen Peng

Utilization of biodiesel by-products for mosquito control
Megha Pant, Satyawati Sharma, Saurabh Dubey, Satya Narayan Naik, Phool Kumar Patanjali

Highlights

・Preparation of biodiesel by-products (karanja and jatropha cake) based mosquito coil formulation.
・Bioefficacy evaluation of the coils against Aedes aegypti adults in terms of repellancy and knockdown effect.
・Confirmation of better performance of the botanicals when used in combination.

Extracellular production of Pseudozyma (Candida) antarctica lipase B with genuine primary sequence in recombinant Escherichia coli
Ayana Ujiie, Hideo Nakano, Yugo Iwasaki

Highlights

・Pseudozyma (Candida) antarctica lipase B (CALB) was produced extracellularly by E. coli.
・The target in the culture supernatant amounted to 550 mg/L.
・5.67 mg of the purified CALB was obtained from 50 mL culture.
・The structural and catalytic integrity of the recombinant CALB was confirmed.
・This is the first example of E. coli-based extracellular production of CALB with the correct primary sequence.

Investigating the role of co-substrate–substrate ratio and filter media on the performance of anammox hybrid reactor treating nitrogen rich wastewater
Swati Tomar, Sunil Kumar Gupta

Highlights

・Filter media (FM) contributed 15.4% additional NH4–N removal.
・FM also contributed 29% reduction in sludge washout rate.
・Mass balance revealed major fraction of input nitrogen got converted into N2 gas.
・Modified Stover–Kincannon model was most suitable for substrate removal kinetics.
・Lawrence and McCarty model suitably predicted bacterial growth kinetics.

Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures
Joon Serk Seo, Byung Sub Min, Young-Bum Kwon, Soo-Young Lee, Jong-Moon Cho, Keun-Hee Park, Yae Ji Yang, Ki Eun Maeng, Shin-Jae Chang, Dong-Il Kim

Oriented growth and transdifferentiation of mesenchymal stem cells towards a Schwann cell fate on micropatterned substrates
Anup D. Sharma, Svitlana Zbarska, Emma M. Petersen, Mustafa E. Marti, Surya K. Mallapragada, Donald S. Sakaguchi

Highlights

・Mesenchymal stem cells were successfully transdifferentiated to Schwann cell-like cells on micropatterned substrates.
・A high degree of transdifferentiation was observed on the polymeric substrates.
・Micropatterns significantly affected the morphology, elongation and orientation of the transdifferentiated cells.
・Micropatterns did not have any effect on cellular area of the cells as compared to cells growing on smooth substrates.
・Patterned films do not cause any deleterious effect on differentiation potential of cells.

Vascular-like network prepared using hollow hydrogel microfibers
Takayuki Takei, Zyunpei Kitazono, Yoshihiro Ozuno, Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida

Cytotoxicity of gold nanoparticles in human neural precursor cells and rat cerebral cortex
Uhn Lee, Chan-Jong Yoo, Yong-Jung Kim, Young-Mi Yoo

Isolation and analysis of polysaccharide showing high hyaluronidase inhibitory activity in Nostochopsis lobatus MAC0804NAN
Yuji Yamaguchi, Mamoru Koketsu

Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis
Yutaka Konya, Takeshi Bamba, Eiichiro Fukusaki