Enhanced production of secretory glycoprotein VSTM1-v2 with mouse IgGκ signal peptide in optimized HEK293F transient transfection
Huihui Liu, Xiajuan Zou, Ting Li, Xiaolin Wang, Wanqiong Yuan, Yingyu Chen, Wenling Han

Highlights

・Mouse IgGκ signal peptide greatly enhanced secretion capacity of VSTM1-v2.
・The yield of VSTM1-v2 in the optimized suspension HEK293F transient expression system was increased by 7 folds.
・VSTM1-v2 was synthesized and secreted as a hyper-glycosylated protein with complex oligosaccharides.
・The purified VSTM1-v2 exhibited excellent bioactivity.
・The study provides a high-efficient way to rapidly enhance production of proteins with weak secretion capacity.

Low-temperature-active and salt-tolerant β-mannanase from a newly isolated Enterobacter sp. strain N18
Jia You, Jin-Feng Liu, Shi-Zhong Yang, Bo-Zhong Mu

Highlights

・We described a high-active and stable extracellular β-mannanase produced by a novel mannanase-producer Enterobacter sp. strain N18.
・The mannanase-producer was obtained by high-throughput screening method.
・The β-mannanase is able to decrease the viscosity of guar gum solution in a very short time which implies the potential for serving as a gel-breaker.
・The β-mannanase is characterized by a broad working temperature especially low temperature, salt-tolerance and wide working pH range.

Immobilization of proteins onto microbeads using a DNA binding tag for enzymatic assays
Takaaki Kojima, Takuro Mizoguchi, Eri Ota, Jumpei Hata, Keisuke Homma, Bo Zhu, Kiyotaka Hitomi, Hideo Nakano

Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium
Nguyen Duc Huy, Cu Le Nguyen, Han-Sung Park, Nguyen Hoang Loc, Myoung-Suk Choi, Dae-Hyuk Kim, Jeong-Woo Seo, Seung-Moon Park

Characterization and evolution of xylose isomerase screened from the bovine rumen metagenome in Saccharomyces cerevisiae
Jin Hou, Yu Shen, Chunlei Jiao, Ruilei Ge, Xingjing Zhang, Xiaoming Bao

Highlights

・Xylose isomerases genes were screened for efficient expression in Saccharomyces cerevisiae.
・The xylose isomerase from bovine rumen exhibited high activity when expressing in S. cerevisiae.
・A variant containing two mutations (K11T/D220V) of Ru-XI that exhibited 68% increase in enzyme activity was isolated.

Simultaneous production of acetic and gluconic acids by a thermotolerant Acetobacter strain during acetous fermentation in a bioreactor
Majid Mounir, Rasoul Shafiei, Raziyeh Zarmehrkhorshid, Allal Hamouda, Mustapha Ismaili Alaoui, Philippe Thonart

Effects of phosphoenolpyruvate carboxylase desensitization on glutamic acid production in Corynebacterium glutamicum ATCC 13032
Masaru Wada, Kazunori Sawada, Kotaro Ogura, Yuta Shimono, Takuya Hagiwara, Masakazu Sugimoto, Akiko Onuki, Atsushi Yokota

Insights into copper effect on Proteus hauseri through proteomic and metabolic analyses
I-Son Ng, Chiming Ye, Yuzhe Li, Bor-Yann Chen

Production of d-psicose from d-fructose by whole recombinant cells with high-level expression of d-psicose 3-epimerase from Agrobacterium tumefaciens
Chang-Su Park, Chul-Soon Park, Kyung-Chul Shin, Deok-Kun Oh

Nitrate removal from high strength nitrate-bearing wastes in granular sludge sequencing batch reactors
Tulasi Venkata Krishna Mohan, Kadali Renu, Yarlagadda Venkata Nancharaiah, Pedapati Murali Satya Sai, Vayalam Purath Venugopalan

Characterization of a halotolerant acetoclastic methanogen highly enriched from marine sediment and its application in removal of acetate
Akihisa Kita, Kazuhito Suehira, Toyokazu Miura, Yoshiko Okamura, Tsunehiro Aki, Yukihiko Matsumura, Takahisa Tajima, Naomichi Nishio, Yutaka Nakashimada

Biological wastewater treatment of 1,4-dioxane using polyethylene glycol gel carriers entrapping Afipia sp. D1
Kazuichi Isaka, Makiko Udagawa, Yuya Kimura, Kazunari Sei, Michihiko Ike

Highlights

・A novel biological 1,4-dioxane (1,4-D) wastewater treatment system was developed.
・The 1,4-D-degrading bacterium Afipia sp. strain D1 was entrapped in gel cubes.
・A high removal rate of 0.67 kg m−3 day−1 and 99% removal efficiency were achieved.
・Significant 1,4-D removal activity was observed even at a low temperature of 6.9°C.
・The activation energy for dioxane degradation was calculated to be 47.3 kJ mol−1.

Cultivation of oleaginous Rhodotorula mucilaginosa in airlift bioreactor by using seawater
Hong-Wei Yen, Yu-Ting Liao, Yi Xian Liu

Paracrine effect of inflammatory cytokine-activated bone marrow mesenchymal stem cells and its role in osteoblast function
Cheng Li, Guoqiang Li, Meng Liu, Tiantian Zhou, Haibin Zhou

Facilitation of uniform maturation of human retinal pigment epithelial cells through collective movement in culture
Rie Sonoi, Mee-Hae Kim, Masahiro Kino-oka

Non-invasive quality evaluation of confluent cells by image-based orientation heterogeneity analysis
Kei Sasaki, Hiroto Sasaki, Atsuki Takahashi, Siu Kang, Tetsuya Yuasa, Ryuji Kato

Metabolic changes in Citrus leaf volatiles in response to environmental stress
Tomonori Asai, Tetsuya Matsukawa, Shin'ichiro Kajiyama

Quantitative analysis of protein orientation in membrane environments by kinase activity
Wen Xiong, Chunshan Quan, Xuning Zhang, Lina Wang, Baoquan Liu, Liming Jin, Shengdi Fan