Novel process for producing 6-deoxy monosaccharides from l-fucose by coupling and sequential enzymatic method
Sirinan Shompoosang, Akihide Yoshihara, Keiko Uechi, Yasuhiko Asada, Kenji Morimoto

Biochemical characterization of a novel thermophilic α-galactosidase from Talaromyces leycettanus JCM12802 with significant transglycosylation activity
Caihong Wang, Huimin Wang, Rui Ma, Pengjun Shi, Canfang Niu, Huiying Luo, Peilong Yang, Bin Yao

Highlights

・A GH27 α-galactosidase was identified in T. leycettanus and expressed in P. pastoris.
・The recombinant Aga27A shows maximum activity at 70°C and is thermotolerant at 65°C.
・It's acidic with optimal activity at 4.0 and keeps stable over pH 3.0–11.0 at 70°C.
・It has strong resistance to most metal ions and great tolerance to galactose.
・It shows the preferable transglycosylation capacity with various substrates.

Inhibitory effect of apocarotenoids on the activity of tyrosinase: Multi-spectroscopic and docking studies
Amrita Anantharaman, Hridya Hemachandran, Rajendra Rao Priya, Mohan Sankari, Mohan Gopalakrishnan, Nallasamy Palanisami, Ramamoorthy Siva

Highlights

・The effective usage of food colorant apocarotenoids as an alternative to synthetic anti-browning agent.
・Apocarotenoids reversibly inhibited tyrosinase activity through extensive hydrophobic interaction.
・The allosteric binding of apocarotenoids to tyrosinase.
・In vitro studies showed that apocarotenoids inhibited melanin synthesis and tyrosinase activity in B16F0 cell line.

Cloning and characterization of GST fusion tag stabilized large subunit of Escherichia coli acetohydroxyacid synthase I
Heng Li, Nan Liu, Wen-Ting Wang, Ji-Yu Wang, Wen-Yun Gao

Heterologously expressed Aspergillus aculeatus β-glucosidase in Saccharomyces cerevisiae is a cost-effective alternative to commercial supplementation of β-glucosidase in industrial ethanol production using Trichoderma reesei cellulases
Treesukon Treebupachatsakul, Hikaru Nakazawa, Hideaki Shinbo, Hiroki Fujikawa, Asami Nagaiwa, Nobuhiro Ochiai, Takashi Kawaguchi, Mitsuru Nikaido, Kazuhide Totani, Koki Shioya, Yosuke Shida, Yasushi Morikawa, Wataru Ogasawara, Hirofumi Okada

Fucoidan from sea cucumber Cucumaria frondosa exhibits anti-hyperglycemic effects in insulin resistant mice via activating the PI3K/PKB pathway and GLUT4
Yiming Wang, Jingfeng Wang, Yanlei Zhao, Shiwei Hu, Di Shi, Changhu Xue

Simple differentiation of sake (Japanese alcoholic beverage) based on trace inorganic components using colorimetric methods
Takashi Kuribayashi, Keigo Sato, Toshio Joh, Mitsuoki Kaneoke, Ken-ichi Watanabe

Polyhydroxyalkanoate production potential of heterotrophic bacteria in activated sludge
Daisuke Inoue, Yuta Suzuki, Takahiro Uchida, Jota Morohoshi, Kazunari Sei

Characterization and flocculability of a novel proteoglycan produced by Talaromyces trachyspermus OU5
Di Fang, Cuicui Shi

Evaluation of bacterial communities by bacteriome analysis targeting 16S rRNA genes and quantitative analysis of ammonia monooxygenase gene in different types of compost
Rika Kitamura, Kazuo Ishii, Isamu Maeda, Toshinori Kozaki, Kazunori Iwabuchi, Takahiro Saito

Impact of zinc supplementation on the improved fructose/xylose utilization and butanol production during acetone–butanol–ethanol fermentation
You-Duo Wu, Chuang Xue, Li-Jie Chen, Feng-Wu Bai

Formation of chitosan-fucoidan nanoparticles and their electrostatic interactions: Quantitative analysis
Eun Ju Lee, Kwang-Hee Lim

Highlights

・The proposed relative charge density model is quantitatively validated.
・A criterion to quantitatively determine the size of CFNs is suggested.
・Ion strength of entrapped solution in CFNs is a key factor of the criterion.
・Evidence of nonspecific interaction was confirmed by shrinkage of CFN size.
・Fractions of nonspecifically bound cationic amino groups were quantified.

Modulation of T cell response by Phellinus linteus
Chun-Jung Lin, Hsiu-Man Lien, Hwai-Jeng Lin, Chao-Lu Huang, Min-Chuan Kao, Yu-An Chen, Chien-Kuo Wang, Hsiao-Yun Chang, Yin-Kuang Chang, Hua-Shan Wu, Chih-Ho Lai

l-Lactic acid production from glycerol coupled with acetic acid metabolism by Enterococcus faecalis without carbon loss
Nao Murakami, Mana Oba, Mariko Iwamoto, Yukihiro Tashiro, Takuya Noguchi, Kaori Bonkohara, Mohamed Ali Abdel-Rahman, Takeshi Zendo, Mitsuya Shimoda, Kenji Sakai, Kenji Sonomoto

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae
Kengo Sasaki, Kiyotaka Y. Hara, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies
Isaac Vizcaino-Caston, Catherine A. Kelly, Annabel V.L. Fitzgerald, Gary A. Leeke, Mike Jenkins, Tim W. Overton

Effect of separation distance on the growth and differentiation of mouse embryoid bodies in micropatterned cultures
Daisuke Miyamoto, Kyohei Ohno, Takuya Hara, Haruka Koga, Kohji Nakazawa

Novel control of gel fraction and enhancement of bonding strength for constructing 3D architecture of tissue engineering scaffold with alginate tubular fiber
Yu Li, Yuanyuan Liu, Shuai Li, Gang Liang, Chen Jiang, Qingxi Hu

Comparisons of cell culture medium using distribution of morphological features in microdevice
Hiroto Sasaki, Junko Enomoto, Yurika Ikeda, Hiroyuki Honda, Junji Fukuda, Ryuji Kato

In vitro probiotic profile based selection of indigenous actinobacterial probiont Streptomyces sp. JD9 for enhanced broiler production
Selvanathan Latha, Gopal Vinothini, Devadasan John Dickson Calvin, Dharumadurai Dhanasekaran