Secretory expression of a phospholipase A2 from Lactobacillus casei DSM20011 in Kluyveromyces lactis
Hui Wang, Liang Zhang, Guiyang Shi

29-Deoxymaklamicin, a new maklamicin analogue produced by a genetically engineered strain of Micromonospora sp. NBRC 110955
Ratama Daduang, Shigeru Kitani, Yuri Sudoh, Ivy Grace Umadhay Pait, Arinthip Thamchaipenet, Haruo Ikeda, Yasuhiro Igarashi, Takuya Nihira

Purification and enzymatic characterization of secretory glycoside hydrolase family 3 (GH3) aryl β-glucosidases screened from Aspergillus oryzae genome
Kanako Kudo, Akira Watanabe, Seiryu Ujiie, Takahiro Shintani, Katsuya Gomi

In vitro catalytic activity of N-terminal and C-terminal domains in NukM, the post-translational modification enzyme of nukacin ISK-1
Chinatsu Shimafuji, Megumi Noguchi, Mami Nishie, Jun-ichi Nagao, Kouki Shioya, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

Photosensitizer and polycationic peptide-labeled streptavidin as a nano-carrier for light-controlled protein transduction
Kosuke Minamihata, Yasukazu Maeda, Satoshi Yamaguchi, Wataru Ishihara, Akira Ishiwatari, Satoshi Takamori, Shinya Yamahira, Teruyuki Nagamune

Heterologous expression of a fungal sterol esterase/lipase in different hosts: Effect on solubility, glycosylation and production
Maria Eugenia Vaquero, Jorge Barriuso, Francisco Javier Medrano, Alicia Prieto, Maria Jesus Martinez

Cloning, expression and characterization of a novel GH5 exo/endoglucanase of Thermobifida halotolerans YIM 90462T by genome mining
Feng Zhang, Xiao-Mei Zhang, Yi-Rui Yin, Wen-Jun Li

Protection of Lactobacillus acidophilus NRRL-B 4495 under in vitro gastrointestinal conditions with whey protein/pullulan microcapsules
Burcu Qabuk, Sebnem Tellioglu Harsa

Highlights

• Effect of pullulan in the encapsulation wall matrix of probiotic L. acidophilus was studied.
• WPI-pullulan encapsulation obtained 93.72% encapsulation efficiency.
• Presence of pullulan provided formation of smoother surfaced microcapsules.
• Release of encapsulated bacteria in SIJ was completed within 7 h.
• WP-pullulan microcapsules obtained sufficient a viable number at the end of SGJ and bile exposure.

Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus β-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass
Treesukon Treebupachatsakul, Koki Shioya, Hikaru Nakazawa, Takashi Kawaguchi, Yasushi Morikawa, Yosuke Shida, Wataru Ogasawara, Hirofumi Okada

Finding of thiosulfate pathway for synthesis of organic sulfur compounds in Saccharomyces cerevisiae and improvement of ethanol production
Eri Funahashi, Kyohei Saiki, Kurara Honda, Yuki Sugiura, Yusuke Kawano, Iwao Ohtsu, Daisuke Watanabe, Yukari Wakabayashi, Tetsuya Abe, Tsuyoshi Nakanishi, Makoto Suematsu, Hiroshi Takagi

Inhibitory effects of sulfur compounds on methane oxidation by a methane-oxidizing consortium
Eun-Hee Lee, Kyung-Eun Moon, Tae Gwan Kim, Sang-Don Lee, Kyung-Suk Cho

Enrichment and specific quantification of Methanocalculus in anaerobic digestion
Si Chen, Qiang He

Highlights

• For the first time Methanocalculus was enriched from anaerobic digestion processes.
• Methanocalculus was enriched under hydrogenotrophic methanogenesis condition only.
• We subsequently developed the first Methanocalculus-specific qPCR assay.
• The qPCR assay was shown to have high coverage, specificity, and efficiency.

Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation
Yu Kiat Lin, Pau Loke Show, Yee Jiun Yap, Chin Ping Tan, Eng-Poh Ng, Arbakariya B. Ariff, Mohamad Suffian B. Mohamad Annuar, Tau Chuan Ling

Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development
Yaya Liu, Weiyan Zhang, Xiancun Deng, Hong Fai Poon, Xuping Liu, Wen-Song Tan, Yan Zhou, Li Fan

Highlights

• A two step strategy for optimizing a chemical defined media is proposed.
• This strategy is proven to be efficient and universal in our current study.
• This strategy identifies potential critical factors in our bioprocess.
• Understanding interaction between physiochemical environment and biological process.

Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway
Yoshikatsu Sato, Nagisa Sugimoto, Tetsuya Higashiyama, Hideyuki Arata

Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells
Rima Matsuyama, Tomomi Tsutsui, Kyoung Ho Lee, Masayoshi Onitsuka, Takeshi Omasa

Changes in human mesenchymal stem cell behaviors on dendrimer-immobilized surfaces due to mediation of fibronectin adsorption and assembly
Yuuki Ogawa, Mee-Hae Kim, Masahiro Kino-oka

Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice
Mai Yoshimoto, Takahiro Otsuki, Kohei Itagaki, Tatsuya Kato, Tetsuya Kohsaka, Yumino Matsumoto, Kazunori Ike, Enoch Y. Park

Protein-poly(amino acid) precipitation stabilizes a therapeutic protein l-asparaginase against physicochemical stress
Takuya Maruyama, Shunsuke Izaki, Takaaki Kurinomaru, Kenji Handa, Tomoaki Kimoto, Kentaro Shiraki

Metabolic characterization of cultured mammalian cells by mass balance analysis, tracer labeling experiments and computer-aided simulations
Nobuyuki Okahashi, Susumu Kohno, Shunsuke Kitajima, Fumio Matsuda, Chiaki Takahashi, Hiroshi Shimizu

Simple and efficient isolation of cordycepin from culture broth of a Cordyceps militaris mutant
Mina Masuda, Masanori Hatashita, Shinya Fujihara, Yu Suzuki, Akihiko Sakurai

Highlights

• The culture broth of the Cordycepin militaris mutant G81-3 had high cordycepin concentration.
• Cordycepin was efficiently isolated from the culture broth by crystallization.
• The combination of temperature and pH shifts was the most effective crystallizing way.
• The purification by crystallization provided a highly purified cordycepin with a high yield.

Corrigendum to “Efficient folding/assembly in CHO cells is critical for high quality (low aggregate content) of secreted trastuzumab as well as for high production: Stepwise multivariate regression analyses” [J Biosci Bioeng 118 (2014) 223–230]
Yoichi Ishii, Junko Murakami, Kazue Sasaki, Masayoshi Tsukahara, Kaori Wakamatsu