Challenges to production of antibodies in bacteria and yeast
Yong Jae Lee, Ki Jun Jeong

Comparison of genetic structures and biochemical properties of tandem cutinase-type polyesterases from Thermobifida alba AHK119
Uschara Thumarat, Takeshi Kawabata, Maho Nakajima, Hajime Nakajima, Akifumi Sugiyama, Kazufumi Yazaki, Tomoko Tada, Tomonori Waku, Naoki Tanaka, Fusako Kawai

Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae
Ryoko Satake, Atsushi Ichiyanagi, Keiichi Ichikawa, Kozo Hirokawa, Yasuko Araki, Taro Yoshimura, Keiko Gomi

Highlights

・A novel FAD-dependent glucose dehydrogenase was identified from Mucor prainii.
・The enzyme showed strict substrate specificity for glucose.
・The effect of additive saccharides for the reactivity was minimal.
・The enzyme was likely suitable for glucose sensor applications.

Optimal fusion of antibody binding domains resulted in higher affinity and wider specificity
Jinhua Dong, Tomoki Kojima, Hiroyuki Ohashi, Hiroshi Ueda

Highlights

・A series of antibody binding proteins PAxPG is made from protein A and protein G.
・Linkage of the domains by a flexible linker yielded higher affinity than the parents.
・Optimization of the linker length resulted in higher affinity to hIgG Fab and Fc.
・With its high affinity, PAxPG will be a useful probe to many Abs including mIgG1.

Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria
Sayaka Hondo, Masatoshi Takahashi, Takashi Osanai, Mami Matsuda, Tomohisa Hasunuma, Akio Tazuke, Yoichi Nakahira, Shigeru Chohnan, Morifumi Hasegawa, Munehiko Asayama

Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae
Akihiro Mori, Shoichi Hara, Tomohiro Sugahara, Takaaki Kojima, Yugo Iwasaki, Yasuaki Kawarasaki, Takehiko Sahara, Satoru Ohgiya, Hideo Nakano

Optimization of cultural conditions for biosurfactant production by Pleurotus djamor in solid state fermentation
Zulfiye Velioglu, Raziye Ozturk Urek

13C-metabolic flux analysis in S-adenosyl-l-methionine production by Saccharomyces cerevisiae
Kenshi Hayakawa, Shuichi Kajihata, Fumio Matsuda, Hiroshi Shimizu

Effects of recombinant lycopene dietary supplement on the egg quality and blood characteristics of laying quails
Wei-Ting Hsu, Chung-Jen Chiang, Yun-Peng Chao, Chi-Huan Chang, Li-Jen Lin, Bi Yu, Tzu-Tai Lee

The isolation and characterization of the novel chlorothalonil-degrading strain Paracoccus sp. XF-3 and the cloning of the chd gene
Wenlong Yue, Minghua Xiong, Feng Li, Guangli Wang

Heterotrophic nitrogen removal by Acinetobacter sp. Y1 isolated from coke plant wastewater
YuXiang Liu, Tingting Hu, Yujie Song, Hongping Chen, YongKang Lv

Application of gas chromatography/flame ionization detector-based metabolite fingerprinting for authentication of Asian palm civet coffee (Kopi Luwak)
Udi Jumhawan, Sastia Prama Putri, Yusianto, Takeshi Bamba, Eiichiro Fukusaki

Partial inhibition of differentiation associated with elevated protein levels of pluripotency factors in mouse embryonic stem cells expressing exogenous EGAM1N homeoprotein
Shiori Sato, Masato Nakazawa, Yumi Kihara, Yusuke Kubo, Yuki Sato, Takahiro Kikuchi, Asumi Nonaka, Akira Sasaki, Jun Iwashita, Jun Murata, Masahiro Hosaka, Masayuki Kobayashi

Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineering
Hirokazu Akiyama, Asako Kobayashi, Masaki Ichimura, Hiroshi Tone, Masaru Nakatani, Minoru Inoue, Arinobu Tojo, Hideaki Kagami

Fabrication of uniformly cell-laden porous scaffolds using a gas-in-liquid templating technique
Takayuki Takei, Ryuta Aokawa, Takamasa Shigemitsu, Koei Kawakami, Masahiro Yoshida

Image-based focused counting of dividing cells for non-invasive monitoring of regenerative medicine products
Kei Sasaki, Hirofumi Miyata, Hiroto Sasaki, Siu Kang, Tetsuya Yuasa, Ryuji Kato

Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli
Miyuki Tsukuda, Nobutaka Nakashima, Kentaro Miyazaki

New compounds, nanaomycin F and G, discovered by physicochemical screening from a culture broth of Streptomyces rosa subsp. notoensis OS-3966
Takuji Nakashima, Panitch Boonsnongcheep, Toru Kimura, Masato Iwatsuki, Noriko Sato, Kenichi Nonaka, Sompop Prathanturarug, Yoko Takahashi, Satoshi Omura