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Vol.120 No.4(2015)
Journal of Bioscience and
Bioengineering
 出版社  (社)日本生物工学会

〒565-0871 大阪府吹田市山田丘2番1号
大阪大学工学部内
TEL 06-6876-2731
FAX 06-6879-2034
URL https://www.sbj.or.jp/

Genetics, Molecular Biology, and Gene Engineering

miR-203 downregulates Yes-1 and suppresses oncogenic activity in human oral cancer cells
Seul-Ah Lee, Jae-Sung Kim, Sun-Young Park, Heung-Joong Kim, Sun-Kyoung Yu, Chun Sung Kim, Hong Sung Chun, Jeongsun Kim, Jong-Tae Park, Daesan Go, Do Kyung Kim

Isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis of Cryptococcus humicola response to aluminum stress
Jingjing Zhang, Lei Zhang, Jinkui Qiu, Hongjuan Nian

Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae
Mustapha Aouida, Lixin Li, Ali Mahjoub, Sahar Alshareef, Zahir Ali, Agnieszka Piatek, Magdy M. Mahfouz

Enzymology, Protein Engineering, and Enzyme Technology

Substrate recognition mechanism of Streptomyces phospholipase D and enzymatic measurement of plasmalogen
Yusaku Matsumoto, Daisuke Sugimori

Molecular cloning and characterization of l-methionine γ-lyase from Streptomyces avermitilis
Daizou Kudou, Eri Yasuda, Yoshiyuki Hirai, Takashi Tamura, Kenji Inagaki

Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system
Kounosuke Hayashi, Jae Man Lee, Yusuke Tomozoe, Takahiro Kusakabe, Noriho Kamiya

Microbial Physiology and Biotechnology

Emulsification and antioxidation of biosurfactant extracts from Chinese medicinal herbs fermentation in vitro
Chunyeh Chen, Tachen Lin, Youmin Shieh

Highlights

・The emulsification and antioxidation of potential BECMHF were detected in vitro.
・BECMHF had good DPPH, superoxide scavenging and superoxide dismutase-like activity.
・BECMHF enhanced the antioxidative effect with amphoteric nature of biosurfactant.
・Potential biosurfactant with amphoteric structure proven was analyzed by TLC and NMR.
・The emulsification and antioxidation of BECMHF have food and cosmetic applications.

Metabolomic analysis of acid stress response in Saccharomyces cerevisiae
Riyanto Heru Nugroho, Katsunori Yoshikawa, Hiroshi Shimizu

Metabolic correlation between polyol and energy-storing carbohydrate under osmotic and oxidative stress condition in Moniliella megachiliensis
Yosuke Kobayashi, Hisashi Iwata, Junjiro Yoshida, Jun Ogihara, Jun Kato, Takafumi Kasumi

Environmental Biotechnology

Effects of bentonite and yeast extract as nutrient on decrease in hydraulic conductivity of porous media due to CaCO3 precipitation induced by Sporosarcina pasteurii
Kagan Eryürük, Suyin Yang, Daisuke Suzuki, Iwao Sakaguchi, Arata Katayama

Nitrous oxide emission in autotrophic partial nitritation system: Macro- and microanalyses
Kai Ju, Lei Wang, Yongtao Lv, Xueling Zhang, Rui Miao, Xudong Wang

Effects of sulfide on the integration of denitrification with anaerobic digestion
Zhixuan Yin, Li Xie, Qi Zhou

Highlights

・Anaerobic acidogenesis was accelerated with low S/NO3−–N ratios (0.27–1.08).
・Increases in S/NO3−–N caused a shift from denitrification to DNRA.
・Heterotrophic denitrification and acidogenesis were suppressed with the S/NO3−–N ratios of 1.60.

Biochemical Engineering

High-yield preparation of recombinant human α-thrombin for therapeutic use
Akihiro Meta, Masaki Hirashima, Takayuki Imamura, Ryoichi Kawamura, Kentaro Yano, Kenji Uehara, Toshihiro Nakashima

Culture characteristics of the atmospheric and room temperature plasma-mutated Spirulina platensis mutants in CO2 aeration culture system for biomass production
Yinyee Tan, Mingyue Fang, Lihua Jin, Chong Zhang, He-Ping Li, Xin-Hui Xing

Production of bacterial cellulose membranes in a modified airlift bioreactor by Gluconacetobacter xylinus
Sheng-Chi Wu, Meng-Hsun Li

The starvation tolerance of anammox bacteria culture at 35°C
Xuan Wu, Sitong Liu, Guanlan Dong, Xiaolin Hou

Cell and Tissue Engineering

MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells
Yuki Iwawaki, Noriko Mizusawa, Takeo Iwata, Nobuaki Higaki, Takaharu Goto, Megumi Watanabe, Yoritoki Tomotake, Tetsuo Ichikawa, Katsuhiko Yoshimoto

Highlights

・Compressive force induced expression of miR-146a-5p, miR-210-3p, miR-494-3p, and miR-1247-3p in MC3T3-E1 cells.
・MiR-494-3p inhibited cell proliferation in MC3T3-E1 cells.
・FGFR2 and ROCK1 were targets of miR-494-3p in MC3T3-E1 cells.

DNA damage-responsive transgene expression mediated by the p53 promoter with transcriptional amplification
Akihiko Ono, Akira Ito, Taiga Suzuki, Masaki Yamaguchi, Yoshinori Kawabe, Masamichi Kamihira

Biomedical Engineering

Suppression of antibiotic resistance acquisition by combined use of antibiotics
Shingo Suzuki, Takaaki Horinouchi, Chikara Furusawa

Methods

Synergism of regulatory elements in σB- and σA-dependent promoters enhances recombinant protein expression in Bacillus subtilis
Teva Phanaksri, Plearnpis Luxananil, Sakol Panyim, Witoon Tirasophon

Lipidomic analysis of plasma lipoprotein fractions in myocardial infarction-prone rabbits
Hiroaki Takeda, Tomonari Koike, Yoshihiro Izumi, Takayuki Yamada, Masaru Yoshida, Masashi Shiomi, Eiichiro Fukusaki, Takeshi Bamba


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