Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains
Fantao Kong, Tomohito Yamasaki, Sari Dewi Kurniasih, Liyuan Hou, Xiaobo Li, Nina Ivanova, Shigeru Okada, Takeshi Ohama

Regulation of 3-hydroxyhexanoate composition in PHBH synthesized by recombinant Cupriavidus necator H16 from plant oil by using butyrate as a co-substrate
Shunsuke Sato, Hiroyuki Maruyama, Tetsuya Fujiki, Keiji Matsumoto

Interaction of carbohydrates with alcohol dehydrogenase: Effect on enzyme activity
Swati B. Jadhav, Sandip B. Bankar, Tom Granström, Heikki Ojamo, Rekha S. Singhal, Shrikant A. Survase

Highlights

・Oxidized glucose, starch and pectin inhibited alcohol dehydrogenase.
・Inhibition rate constant increased with an increase in concentration of carbohydrates.
・Thiol groups of enzyme were involved in binding with inhibitiors.
・Oxidized pectin was the most potent inhibitors among the carbohydrates studied.

Enhancement of immobilized lipase activity by design of polymer brushes on a hollow fiber membrane
Tadashi Okobira, Ai Matsuo, Hikaru Matsumoto, Takanori Tanaka, Kazuya Kai, Chie Minari, Muneharu Goto, Hidetaka Kawakita, Kazuya Uezu

Highlights

・We grafted a polymer brush possessing aminoethanol onto a hollow fiber membrane to immobilize lipase.
・The water molecules which AE retained played an important role for keeping activity of lipase.
・We controlled the length and density of the polymer brushes to evaluate the effects to activity of lipase.
・The improved polymer brushes achieved nineteen-fold higher esterification than that of before improvement.

Novel acidophilic β-galactosidase with high activity at extremely acidic pH region from Teratosphaeria acidotherma AIU BGA-1
Serina Chiba, Miwa Yamada, Kimiyasu Isobe

Sequence modifications and heterologous expression of eukaryotic cytochromes P450 in Escherichia coli
Hirofumi Ichinose, Mayumi Hatakeyama, Yukiyo Yamauchi

Inactivation of Shiga toxin-producing Escherichia coli O104:H4 using cold atmospheric pressure plasma
Matthias Baier, Traute Janßen, Lothar H. Wieler, Jörg Ehlbeck, Dietrich Knorr, Oliver Schlüter

Absolute quantitation of glycolytic intermediates reveals thermodynamic shifts in Saccharomyces cerevisiae strains lacking PFK1 or ZWF1 genes
Shunsuke Nishino, Nobuyuki Okahashi, Fumio Matsuda, Hiroshi Shimizu

Response of Fe–S cluster assembly machinery of Escherichia coli to mechanical stress in a model of amino-acid crystal fermentation
Satoshi Okutani, Takayoshi Iwai, Shintaro Iwatani, Kiyoshi Matsuno, Yasuhiro Takahashi, Toshiharu Hase

Ergothioneine protects Streptomyces coelicolor A3(2) from oxidative stresses
Shunsuke Nakajima, Yasuharu Satoh, Kentaro Yanashima, Tomomi Matsui, Tohru Dairi

Omega-3 eicosatetraenoic acid production by molecular breeding of the mutant strain S14 derived from Mortierella alpina 1S-4
Tomoyo Okuda, Akinori Ando, Hiroaki Negoro, Hiroshi Kikukawa, Takaiku Sakamoto, Eiji Sakuradani, Sakayu Shimizu, Jun Ogawa

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant Escherichia coli from glucose
Ayaka Hokamura, Izumi Wakida, Yuki Miyahara, Takeharu Tsuge, Hideki Shiratsuchi, Kenji Tanaka, Hiromi Matsusaki

Determination and quantification of kokumi peptide, γ-glutamyl-valyl-glycine, in brewed alcoholic beverages
Naohiro Miyamura, Yuko Iida, Motonaka Kuroda, Yumiko Kato, Junko Yamazaki, Toshimi Mizukoshi, Hiroshi Miyano

Biogas production from chicken manure at different organic loading rates in a mesophilic-thermopilic two stage anaerobic system
Kenan Dalkilic, Aysenur Ugurlu

Optimization of cell line development in the GS-CHO expression system using a high-throughput, single cell-based clone selection system
Tsuyoshi Nakamura, Takeshi Omasa

Highlights

・We applied the high-throughput ClonePix FL for cell line development using CHO-K1SV.
・We conducted optimization of the semi-solid medium formulation for CHO-K1SV.
・We showed the importance of spent medium and recombinant Albumine in semi-solid media.
・The cell culture duration after transfection affected the colony formation ratio.
・Considerations of optimized conditions are seemed to be useful for other cell lines.

Immobilization of feruloyl esterases on magnetic nanoparticles and its potential in production of ferulic acid
Fuming He, Song Zhang, Xinli Liu

Effect of kojic acid-grafted-chitosan oligosaccharides as a novel antibacterial agent on cell membrane of gram-positive and gram-negative bacteria
Xiaoli Liu, Wenshui Xia, Qixing Jiang, Yanshun Xu, Peipei Yu

Highlights

・The modes of action of COS/KA on Gram-positive and Gram-negative bacteria were examined.
・COS/KA increased the membrane permeability by adhering to cell membranes.
・COS/KA resulted in leakage of cell inclusions (enzymes, DNA, proteins and ions).
・Gram-positive bacteria were more sensitive than Gram-negative bacteria to COS/KA.
・COS/KA has potential in food industry and biomedical sciences.

Efficient enrichment of high-producing recombinant Chinese hamster ovary cells for monoclonal antibody by flow cytometry
Takeshi Okumura, Kenji Masuda, Kazuhiko Watanabe, Kenji Miyadai, Koichi Nonaka, Masayuki Yabuta, Takeshi Omasa

Vessel network formation in response to intermittent hypoxia is frequency dependent
Seema M. Ehsan, Steven C. George