Characterization of a novel thermostable N-acylhomoserine lactonase from the thermophilic bacterium Thermaerobacter marianensis
Tomohiro Morohoshi, Yoshiaki Tominaga, Nobutaka Someya, Tsukasa Ikeda

Improving xylitol production through recombinant expression of xylose reductase in the white-rot fungus Phanerochaete sordida YK-624
Sho Hirabayashi, Jianqiao Wang, Hirokazu Kawagishi, Hirofumi Hirai

Glucose oxidase adsorption performance of carbonaceous mesocellular foams prepared with different carbon sources
Fengjiao Zhou, Ximing Pu, Dapeng Luo, Guangfu Yin, Kai Zhuang, Xiaoming Liao, Zhongbin Huang, Xianchun Chen, Yadong Yao

Enzyme-assisted supercritical carbon dioxide extraction of black pepper oleoresin for enhanced yield of piperine-rich extract
Sayantani Dutta, Paramita Bhattacharjee

Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa: Screening, molecular genetic analysis, expression and characterization
Noriyuki Doukyu, Shyou Nihei

Requirement of carbon dioxide for initial growth of facultative methylotroph, Acidomonas methanolica MB58
Ryoji Mitsui, Hiroko Katayama, Mitsuo Tanaka

Enhancement of l-phenylalanine production by engineered Escherichia coli using phased exponential l-tyrosine feeding combined with nitrogen source optimization
Peipei Yuan, Weijia Cao, Zhen Wang, Kequan Chen, Yan Li, Pingkai Ouyang

Selection of suitably non-repressing carbon sources for expression of alcohol oxidase isozyme promoters in the methylotrophic yeast Pichia methanolica
Tomoyuki Nakagawa, Keishi Wakayama, Takashi Hayakawa

Evaluation of the effects of amyloid β aggregation from seaweed extracts by a microliter-scale high-throughput screening system with a quantum dot nanoprobe
Toshiki Ogara, Tomohito Takahashi, Hajime Yasui, Koji Uwai, Kiyotaka Tokuraku

Anaerobic co-digestion of kitchen waste and pig manure with different mixing ratios
Hailin Tian, Na Duan, Cong Lin, Xue Li, Mingzhu Zhong

Characterization of water-soluble dark-brown pigment from Antarctic bacterium, Lysobacter oligotrophicus
Tomomi Kimura, Wakao Fukuda, Tomoe Sanada, Tadayuki Imanaka

Polyphasic characterization of an anaerobic hexachlorobenzene-dechlorinating microbial consortium with a wide dechlorination spectrum for chlorobenzenes
Xue Zhou, Chunfang Zhang, Dongdong Zhang, Takanori Awata, Zhixing Xiao, Qi Yang, Arata Katayama

Genome sequence determination and metagenomic characterization of a Dehalococcoides mixed culture grown on cis-1,2-dichloroethene
Masafumi Yohda, Osami Yagi, Ayane Takechi, Mizuki Kitajima, Hisashi Matsuda, Naoaki Miyamura, Tomoko Aizawa, Mutsuyasu Nakajima, Michio Sunairi, Akito Daiba, Takashi Miyajima, Morimi Teruya, Kuniko Teruya, Akino Shiroma, Makiko Shimoji, Hinako Tamotsu, Ayaka Juan, Kazuma Nakano, Misako Aoyama, Yasunobu Terabayashi, Kazuhito Satou, et al.

Optimization of chemically defined feed media for monoclonal antibody production in Chinese hamster ovary cells
Shohei Kishishita, Satoshi Katayama, Kunihiko Kodaira, Yoshinori Takagi, Hiroki Matsuda, Hiroshi Okamoto, Shinya Takuma, Chikashi Hirashima, Hideki Aoyagi

Characterization of bovine serum albumin partitioning behaviors in polymer-salt aqueous two-phase systems
Yin Hui Chow, Yee Jiun Yap, Chin Ping Tan, Mohd Shamsul Anuar, Bimo Ario Tejo, Pau Loke Show, Arbakariya Bin Ariff, Eng-Poh Ng, Tau Chuan Ling

Highlights

• A linear relationship between ln K and TLL2 is proposed.
• A linear relationship between ln K and pH is proposed.
• Results showed that the equations proposed give good fits for all the ATPS studied.

In situ chondrogenic differentiation of bone marrow stromal cells in bioactive self-assembled peptide gels
Ji Eun Kim, Soo Hyun Kim, Youngmee Jung

Improved transgene integration into the Chinese hamster ovary cell genome using the Cre-loxP system
Takanori Inao, Yoshinori Kawabe, Takuro Yamashiro, Yujiro Kameyama, Xue Wang, Akira Ito, Masamichi Kamihira

Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay
Masayoshi Onitsuka, Takeshi Omasa

Highlights

• Rapid and high-throughput detection for antibody glycosylation.
• The detection is based on chemiluminescent-bead-based lectin binding.
• This method is applicable to evaluation of glycosylation dynamics in cell culture.
  

Preparation of keratin and chemically modified keratin hydrogels and their evaluation as cell substrate with drug releasing ability
Ryo Nakata, Yu Osumi, Shoko Miyagawa, Akira Tachibana, Toshizumi Tanabe

Highlights

• A hydrogel of reduced keratin was prepared simply by concentration and subsequent cooling.
• Hydrogels could be obtained after chemical modification of keratin.
• Cells attached and proliferated well on each hydrogel.
• Keratin and acetamidated keratin hydrogels showed good sustained drug release.

Enzyme assay for pyridoxal 5′-phosphate by apo-d-amino acid aminotransferase
Hiroki Ota, Masuwo Mushiga, Tohru Yoshimura, Kazuaki Yoshimune