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Vol.119 No.4(2015)
Journal of Bioscience and
Bioengineering
 出版社  (社)日本生物工学会

〒565-0871 大阪府吹田市山田丘2番1号
大阪大学工学部内
TEL 06-6876-2731
FAX 06-6879-2034
URL https://www.sbj.or.jp/

Review

Dynamics of the microbial community during continuous methane fermentation in continuously stirred tank reactorsReview Article
Yue-Qin Tang, Toru Shigematsu, Shigeru Morimura, Kenji Kida

Genetics, Molecular Biology, and Gene Engineering

Identification of glycosyl hydrolases from a metagenomic library of microflora in sugarcane bagasse collection site and their cooperative action on cellulose degradation
Pattanop Kanokratana, Lily Eurwilaichitr, Kusol Pootanakit, Verawat Champreda

Type 2C protein phosphatase Ptc6 participates in activation of the Slt2-mediated cell wall integrity pathway in Saccharomyces cerevisiae
Dilruba Sharmin, Yu Sasano, Minetaka Sugiyama, Satoshi Harashima

Production of initial-stage eukaryotic N-glycan and its protein glycosylation in Escherichia coli
Akkaraphol Srichaisupakit, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Gene dynamics of core transcription factors for pluripotency in embryonic stem cells
Brit G. David, Kazuko Okamoto, Taishi Kakizuka, Taro Ichimura, Tomonobu M. Watanabe, Hideaki Fujita

Enzymology, Protein Engineering, and Enzyme Technology

A new aldehyde oxidase catalyzing the conversion of glycolaldehyde to glycolate from Burkholderia sp. AIU 129
Miwa Yamada, Keika Adachi, Natsumi Ogawa, Shigenobu Kishino, Jun Ogawa, Michihiko Kataoka, Sakayu Shimizu, Kimiyasu Isobe

Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase
Nguyen Duc Huy, Cu Le Nguyen, Jeong-Woo Seo, Dae-Hyuk Kim, Seung-Moon Park

Highlights

  • A novel β-xylosidase (PcXyl5) belonging in glycoside hydrolase family 5 was cloned and characterized.
  • rPcXyl5 completely hydrolyzed xylo-oligomers to xylose and performed transglycosylation.
  • rPcXyl5 enhanced xylans and pretreated barley straw saccharification in synergy with endo-xylanase.

Comparative study of fungal strains for thermostable inulinase production
Adriana C. Flores-Gallegos, Juan C. Contreras-Esquivel, Jesús A. Morlett-Chávez, Cristóbal N. Aguilar, Raúl Rodríguez-Herrera

Microbial Physiology and Biotechnology

Enhanced cellular content and lactate fraction of the poly(lactate-co-3-hydroxybutyrate) polyester produced in recombinant Escherichia coli by the deletion of σ factor RpoN
Ryosuke Kadoya, Yu Kodama, Ken'ichiro Matsumoto, Seiichi Taguchi

Brewing and Food Technology

Lactobacillus plantarum mediated fermentation of Psidium guajava L. fruit extract
Ravish Bhat, Lakshminarayana Chikkanayakanahalli Suryanarayana, Karunakara Alageri Chandrashekara, Padma Krishnan, Anil Kush, Puja Ravikumar

Highlights

  • Fermentation of Raw Guava fruit extract by L. plantarum NCIM 2912.
  • 16 hour shake flask fermentation process optimized.
  • Increase in antioxidant activity, total phenolics, short and medium chain fatty acids.
  • Fermented product miscible in water and amorphous powder upon lyophilization.
Environmental Biotechnology

Biosorption of cationic basic dye and cadmium by the novel biosorbent Bacillus catenulatus JB-022 strain
Su Young Kim, Mi Ra Jin, Chang Ho Chung, Yeoung-Sang Yun, Kwang Yeop Jahng, Kang-Yeol Yu

Effects of culture conditions of Pseudomonas aeruginosa strain RB on the synthesis of CdSe nanoparticles
Hiroyuki Ayano, Masashi Kuroda, Satoshi Soda, Michihiko Ike

Biochemical Engineering

Preparation of melanin from Catharsius molossus L. and preliminary study on its chemical structure
Chao Xin, Jia-hua Ma, Cheng-jia Tan, Zhou Yang, Feng Ye, Chan Long, Shuang Ye, Da-bin Hou

Effective stimulating factors for microbial levan production by Halomonas smyrnensis AAD6T
Hande Kazak Sarilmiser, Ozlem Ates, Gonca Ozdemir, Kazim Yalcin Arga, Ebru Toksoy Oner

Plant Biotechnology

Polygonum cuspidatum extracts as bioactive antioxidaion, anti-tyrosinase, immune stimulation and anticancer agents
Chih-Chen Lee, Yen-Ting Chen, Chien-Chih Chiu, Wei-Ting Liao, Yung-Chuan Liu, Hui-Min David Wang

Characterization of cucumber violaxanthin de-epoxidase gene promoter in Arabidopsis
Xin Li, Xiaolei Sui, Wenchao Zhao, Hongyu Huang, Yakun Chen, Zhenxian Zhang

Cell and Tissue Engineeering

Titer of trastuzumab produced by a Chinese hamster ovary cell line is associated with tricarboxylic acid cycle activity rather than lactate metabolism
Yoichi Ishii, Yasufumi Imamoto, Rie Yamamoto, Masayoshi Tsukahara, Kaori Wakamatsu

Highlights

  • Two contrasting cell lines A and B were characterized by metabolomic analysis.
  • High antibody titer in cell line A was due to high TCA cycle activity.
  • Different defucosylation levels were not due to different GDP-fucose levels.
  • Higher covalent aggregate content in cell line A was due to high oxidative stress.

Islets immunoisolation using encapsulation and PEGylation, simultaneously, as a novel design
Mohammad Mahdi Nabavimanesh, Sameereh Hashemi-Najafabadi, Ebrahim Vasheghani-Farahani

Methods

Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device
Hitoyoshi Yamashita, Masamune Morita, Haruka Sugiura, Kei Fujiwara, Hiroaki Onoe, Masahiro Takinoue


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