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Vol.119 No.2(2015)
Journal of Bioscience and
Bioengineering
 出版社  (社)日本生物工学会

〒565-0871 大阪府吹田市山田丘2番1号
大阪大学工学部内
TEL 06-6876-2731
FAX 06-6879-2034
URL https://www.sbj.or.jp/

Enzymology, Protein Engineering, and Enzyme Technology

Characterization of glycerophosphoethanolamine ethanolaminephosphodiesterase from Streptomyces sanglieri
Shingo Mineta, Kazutaka Murayama, Daisuke Sugimori

Improvement of glycosylation structure by suppression of β-N-acetylglucosaminidases in silkworm
Tsuyoshi Nomura, Masatoshi Suganuma, Yukiko Higa, Yukiko Kataoka, Shunsuke Funaguma, Hironobu Okazaki, Takeo Suzuki, Isao Kobayashi, Hideki Sezutsu, Kazuhito Fujiyama

In vitro selection of a photoresponsive peptide aptamer to glutathione-immobilized microbeads
Seiichi Tada, Qingmin Zang, Wei Wang, Masuki Kawamoto, Mingzhe Liu, Michiru Iwashita, Takanori Uzawa, Daisuke Kiga, Masayuki Yamamura, Yoshihiro Ito

Microbial Physiology and Biotechnology

Isolation and characterization of awamori yeast mutants with l-leucine accumulation that overproduce isoamyl alcohol
Hiroshi Takagi, Keisuke Hashida, Daisuke Watanabe, Ryo Nasuno, Masataka Ohashi, Tomoya Iha, Maiko Nezuo, Masatoshi Tsukahara

Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class
Hisashi Iwata, Daiki Mizushima, Yosuke Kobayashi, Tetsuya Ookura, Jun Ogihara, Jun Kato, Takafumi Kasumi

Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression
Mohamed Ali Abdel-Rahman, Yaotian Xiao, Yukihiro Tashiro, Ying Wang, Takeshi Zendo, Kenji Sakai, Kenji Sonomoto

Influence of ofloxacin on photosystems I and II activities of Microcystis aeruginosa and the potential role of cyclic electron flow
Chunnuan Deng, Xiangliang Pan, Daoyong Zhang

Production of squalene by squalene synthases and their truncated mutants in Escherichia coli
Akinori Katabami, Ling Li, Miki Iwasaki, Maiko Furubayashi, Kyoichi Saito, Daisuke Umeno

Trehalose accumulation enhances tolerance of Saccharomyces cerevisiae to acetic acid
Yoko Yoshiyama, Koichi Tanaka, Kohei Yoshiyama, Makoto Hibi, Jun Ogawa, Jun Shima

Enhancement of l-cysteine production by disruption of yciW in Escherichia coli
Yusuke Kawano, Iwao Ohtsu, Kazuhiro Takumi, Ai Tamakoshi, Gen Nonaka, Eri Funahashi, Masaki Ihara, Hiroshi Takagi

Environmental Biotechnology

Autotrophic denitrification performance and bacterial community at biocathodes of bioelectrochemical systems with either abiotic or biotic anodes
Van Khanh Nguyen, Sungsug Hong, Younghyun Park, Kyungmin Jo, Taeho Lee

Humin as an electron donor for enhancement of multiple microbial reduction reactions with different redox potentials in a consortium
Dongdong Zhang, Chunfang Zhang, Zhixing Xiao, Daisuke Suzuki, Arata Katayama

Biochemical Engineering

Reflectometric interference spectroscopy-based immunosensing using immobilized antibody via His-tagged recombinant protein A
Hyung Woo Choi, Yasuhiko Sakata, Tooru Ooya, Toshifumi Takeuchi

Molecularly imprinted protein recognition thin films constructed by controlled/living radical polymerization
Shogo Sasaki, Tooru Ooya, Yukiya Kitayama, Toshifumi Takeuchi

Simultaneous recovery and purification of rice protein and phosphorus compounds from full-fat and defatted rice bran with organic solvent-free process
Masanori Watanabe, Isamu Maeda, Masahiro Koyama, Kozo Nakamura, Kazuo Sasano

Cell and Tissue Engineering

Microfluidic devices for construction of contractile skeletal muscle microtissues
Kazunori Shimizu, Hiroyuki Araki, Kohei Sakata, Wataru Tonomura, Mitsuru Hashida, Satoshi Konishi

Gamma-cross-linked nonfibrillar collagen gel as a scaffold for osteogenic differentiation of mesenchymal stem cells
Takako Takitoh, Masahiko Bessho, Motohiro Hirose, Hajime Ohgushi, Hideki Mori, Masayuki Hara

Highlights

  • We prepared gamma-cross-linked nonfibrillar collagen gels and unirradiated fibrillar gels.
  • The mesenchymal stem cells (MSCs) differentiated into osteoblasts on both collagen gels.
  • The MSC-derived osteoblasts deposited calcium on both collagen gels.
  • Bone formation by osteoblasts was more efficient in the nonfibrillar gels than the fibrillar gels.
  • The MSCs had a unique elongated shape and adhered to each other on the nonfibrillar gels.

Hydrocortisone and triiodothyronine regulate hyaluronate synthesis in a tissue-engineered human dermal equivalent through independent pathways
Madhura Deshpande, Suzanne Papp, Lana Schaffer, Tara Pouyani

Biomedical Engineering

Relative charge density model on chitosan-fucoidan electrostatic interaction: Qualitative approach with element analysis
Eun Ju Lee, Kwang-Hee Lim

Highlights

  • We propose a relative charge density model of chitosan-fucoidan nanoparticles.
  • The model can control the size of chitosan-fucoidan nanoparticles.
  • At pH 2, sulfate group-rich nanoparticles are prepared with a low yield.
  • At pH 6, sulfate group-poor nanoparticles are prepared with a high yield.
  • We obtain separate yield-distributions of each loaded chitosan and fucoidan.
Methods

Simple and reliable urea assay based on a signal accumulation type of ion-sensitive field-effect transistor
Naohiro Tomari, Asako Kawasaki, Yoshihiro Yamamoto, Yoshiaki Nishiya


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