Recent advances to improve fermentative butanol production: Genetic engineering and fermentation technologyReview Article
Jin Zheng, Yukihiro Tashiro, Qunhui Wang, Kenji Sonomoto

Fermentative production of lactic acid from renewable materials: Recent achievements, prospects, and limitsReview Article
Ying Wang, Yukihiro Tashiro, Kenji Sonomoto

Expression and characterization of an M cell-specific ligand-fused dengue virus tetravalent epitope using Saccharomyces cerevisiaeOriginal Research Article
Ngoc-Luong Nguyen, Kum-Kang So, Jung-Mi Kim, Sae-Hae Kim, Yong-Suk Jang, Moon-Sik Yang, Dae-Hyuk Kim

Chloroplast-targeting protein expression in the oleaginous diatom Fistulifera solaris JPCC DA0580 toward metabolic engineeringOriginal Research Article
Yoshihiko Sunaga, Yoshiaki Maeda, Takashi Yabuuchi, Masaki Muto, Tomoko Yoshino, Tsuyoshi Tanaka

Sulforaphane down-regulates SKP2 to stabilize p27KIP1 for inducing antiproliferation in human colon adenocarcinoma cellsOriginal Research Article
Yuan-Kai Chung, Richard Chi-Hung Or, Chien-Hsing Lu, Wei-Ting Ouyang, Shu-Yi Yang, Chia-Che Chang

Telomere-mediated chromosomal truncation in Aspergillus oryzae
Sawaki Tada, Hikaru Ohkuchi, Mayumi Matsushita-Morita, Ikuyo Furukawa, Ryota Hattori, Satoshi Suzuki, Yutaka Kashiwagi, Ken-Ichi Kusumoto

Alteration of the substrate specificity of cytochrome P450 CYP199A2 by site-directed mutagenesisOriginal Research Article
Toshiki Furuya, Yoh Shitashima, Kuniki Kino

Purification and properties of recombinant exopolyphosphatase PPN1 and effects of its overexpression on polyphosphate in Saccharomyces cerevisiaeOriginal Research Article
Nadeshda Andreeva, Ludmila Trilisenko, Tatiana Kulakovskaya, Maria Dumina, Michail Eldarov

Highlights

• ・Saccharomyces cerevisiae strain overexpressing polyphosphatase PPN1 was constructed.
• ・Recombinant PPN1 was purified and characterized.
• ・Amino acid sequence of recombinant PPN1 was the same as in mature wild type PPN1.
• ・The recombinant PPN1 was localized in the cytoplasm and vacuoles.
• ・PPN1 polyphosphatase overexpression decreases short-chain polyphosphate level.

Identification and characterization of d-xylose reductase involved in pentose catabolism of the zygomycetous fungus Rhizomucor pusillusOriginal Research Article
Hidenobu Komeda, Shino Yamasaki-Yashiki, Kazuhiro Hoshino, Yasuhisa Asano

Production of d-lactic acid in a continuous membrane integrated fermentation reactor by genetically modified Saccharomyces cerevisiae: Enhancement in d-lactic acid carbon yieldOriginal Research Article
Takashi Mimitsuka, Kenji Sawai, Koji Kobayashi, Takeshi Tsukada, Norihiro Takeuchi, Katsushige Yamada, Hiroyasu Ogino, Tetsu Yonehara

Highlights

• ・Continuous culture by lactate-producing yeast increased lactate yield.
• ・d-Lactic acid carbon yield increased at a later phase of the batch culture.
• ・Continuous culture keeps the culture condition at the batch’s late stationary phase.
• ・Continuous air supply during fermentation maintains a stable cytosolic redox balance.
• ・Continuous fermentation releases S. cerevisiae from the Crabtree effect.

Microbial transformations of halolactones with p-menthane systemOriginal Research Article
Marcelina Mazur, Aleksandra Grudniewska, Czeslaw Wawrzenczyk

Modulating betulinic acid production in Saccharomyces cerevisiae by managing the intracellular supplies of the co-factor NADPH and oxygenOriginal Research Article
Jing Li, Yansheng Zhang

Effect of malic enzyme on ethanol production by Synechocystis sp. PCC 6803
Katsunori Yoshikawa, Takashi Hirasawa, Hiroshi Shimizu

Inhibitory effect of fucosylated chondroitin sulfate from the sea cucumber Acaudina molpadioides on adipogenesis is dependent on Wnt/β-catenin pathwayOriginal Research Article
Hui Xu, Jingfeng Wang, Xin Zhang, Zhaojie Li, Yuming Wang, Changhu Xue

Expression of key hydrolases for soy sauce fermentation in Zygosaccharomyces rouxii
Masanobu Yuzuki, Kenichiro Matsushima, Yasuji Koyama

Nitrate reduction pathway in an anaerobic acidification reactor and its effect on acid fermentationOriginal Research Article
Li Xie, Chi Ji, Rui Wang, Qi Zhou

Comparative metabolic flux analysis of an Ashbya gossypii wild type strain and a high riboflavin-producing mutant strainOriginal Research Article
Bo-Young Jeong, Christoph Wittmann, Tatsuya Kato, Enoch Y. Park

Locational heterogeneity of maturation by changes in migratory behaviors of human retinal pigment epithelial cells in cultureOriginal Research Article
Rie Sonoi, Mee-Hae Kim, Masahiro Kino-oka

Femtogram detection of horseradish peroxidase by a common desktop scanner
Shahila Parween, Pradip Nahar

Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry
Fumio Matsuda, Tairo Ogura, Atsumi Tomita, Ichiro Hirano, Hiroshi Shimizu

Corrigendum to “Probing of exopolysaccharides with green fluorescence protein-labeled carbohydrate-binding module in Escherichia coli biofilms and flocs induced by bcsB overexpression” [J Biosci Bioeng 118 (2014) 400–405]
Minh Hong Nguyen, Yoshihiro Ojima, Makiko Sakka, Kazuo Sakka, Masahito Taya