Binding affinity of ssDNA is improved by attachment of dsDNA regions
Hiroyuki Ida, Akira Tachibana, Toshizumi Tanabe

Co-expression of a heat shock transcription factor to improve conformational quality of recombinant protein in Escherichia coli
Shao-Yen Hsu, Yu-Sheng Lin, Shu-Jyuan Li, Wen-Chien Lee

Highlights

• Co-expression of heat shock transcription factor sigma 32 in recombinant Escherichia coli.
  
• Higher enzymatic activity of overexpressed protein in both soluble fraction and inclusion body.
  
• Promoting conformational quality of the recombinant protein by the co-expression of sigma 32.
  
• Co-expression led to a decrease in the particle size of inclusion bodies.

Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide
Yi Liu, Wenjing Cui, Zhongmei Liu, Youtian Cui, Yuanyuan Xia, Michihiko Kobayashi, Zhemin Zhou

Analysis of specific proteolytic digestion of the peptidoglutaminase–asparaginase of koji molds
Kotaro Ito, Yasuji Koyama

Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli
Akkaraphol Srichaisupakit, Takao Ohashi, Kazuhito Fujiyama

Importance of glucose-6-phosphate dehydrogenase (G6PDH) for vanillin tolerance in Saccharomyces cerevisiae
Trinh Thi My Nguyen, Sakihito Kitajima, Shingo Izawa

Role of Karanja deoiled cake based medium in production of protease and fatty acids by Paecilomyces lilacinus 6029
Abhishek Sharma, Satyawati Sharma, Savita Yadav, Satya N. Naik

Molecular analysis of Oenococcus oeni and the relationships among and between commercial and autochthonous strains
Lucía González-Arenzana, Rosa López, Javier Portu, Pilar Santamaría, Teresa Garde-Cerdán, Isabel López-Alfaro

Effect of ammonium nitrogen concentration on the ammonia-oxidizing bacteria community in a membrane bioreactor for the treatment of anaerobically digested swine wastewater
Qianwen Sui, Chong Liu, Hongmin Dong, Zhiping Zhu

Effects of increasing organic loading rate on performance and microbial community shift of an up-flow anaerobic sludge blanket reactor treating diluted pharmaceutical wastewater
Zhu Chen, Yuguang Wang, Kai Li, Hongbo Zhou

Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant
Kang Song, Toshikazu Suenaga, Aki Hamamoto, Kouichi Satou, Shohei Riya, Masaaki Hosomi, Akihiko Terada

Orthogonal array deciphering MRS medium requirements for isolated Lactobacillus rhamnosus ZY with cell properties characterization
Yu Zhang, I-Son Ng, Chuanyi Yao, Yinghua Lu

Efficient production of mutant phytase (phyA-7) derived from Selenomonas ruminantium using recombinant Escherichia coli in pilot scale
John Chi-Wei Lan, Chih-Kai Chang, Ho-Shing Wu

Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis
Thomas Hahne, Muthukutty Palaniyandi, Tatsuya Kato, Peter Fleischmann, Hermann Wätzig, Enoch Y. Park

Control of adhesion of human induced pluripotent stem cells to plasma-patterned polydimethylsiloxane coated with vitronectin and γ-globulin
Ryotaro Yamada, Koji Hattori, Saoko Tachikawa, Motohiro Tagaya, Toru Sasaki, Shinji Sugiura, Toshiyuki Kanamori, Kiyoshi Ohnuma

Keratinization induced by air exposure in the reconstructed human epidermal model: An in vitro model of a cultured epithelial autograft
Takao Hanada, Yuichi Itahara, Masakazu Katoh, Masukazu Inoie, Ken-ichiro Hata

Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function
Koji Hattori, Yoichi Munehira, Hideki Kobayashi, Taku Satoh, Shinji Sugiura, Toshiyuki Kanamori

Effect of plasma-irradiated silk fibroin in bone regeneration
Ryoichiro Uchida, Ujjal K. Bhawal, Hideo Kiba, Kiyoshi Arai, Yasuhiro Tanimoto, Noboru Kuboyama, Tetsuo Asakura, Norihiro Nishiyama

Expression of a novel recombinant fusion protein BVN-Tβ4 and its effects on diabetic wound healing
Qingfeng Zhou, Shuna Jiang, Kang Ma, Chengwei Li

System-on-fluidics immunoassay device integrating wireless radio-frequency-identification sensor chips
Yoshiaki Yazawa, Tadashi Oonishi, Kazuki Watanabe, Akiko Shiratori, Sohei Funaoka, Masao Fukushima

Metabolic distance estimation based on principle component analysis of metabolic turnover
Yasumune Nakayama, Sastia P. Putri, Takeshi Bamba, Eiichiro Fukusaki

Microfluidic perfusion cell culture system confined in 35 mm culture dish for standard biological laboratories
Eitaro Kondo, Ken-Ichi Wada, Kazuo Hosokawa, Mizuo Maeda