Purification and characterization of an oxygen-evolving photosystem II from Leptolyngbya sp. strain O-77
Harutaka Nakamori, Takeshi Yatabe, Ki-Seok Yoon, Seiji Ogo

Purification and characterization of a novel β-agarase of Paenibacillus sp. SSG-1 isolated from soil
Tao Song, Yu Cao, Hui Xu, Weijia Zhang, Baojin Fei, Dairong Qiao, Yi Cao

Incorporation of adenine nucleotide transporter, Ant1p, into proteoliposomes facilitates ATP translocation and activation of encapsulated luciferase
Akira Nozawa, Yuzuru Tozawa

Pellicle of thermotolerant Acetobacter pasteurianus strains: Characterization of the polysaccharides and of the induction patterns
Perumpuli Arachchige Buddhika Niroshie Perumpuli, Taisuke Watanabe, Hirohide Toyama

Improvement of organic solvent tolerance by disruption of the lon gene in Escherichia coli
Rei Watanabe, Noriyuki Doukyu

Production and characterization of medium-chain-length polyhydroxyalkanoates by Pseudomonas mosselii TO7
Yi-Jr Chen, Yan-Chia Huang, Chia-Yin Lee

Isolation of potential probiotic Lactobacillus oris HMI68 from mother's milk with cholesterol-reducing property
Marimuthu Anandharaj, Balayogan Sivasankari

Highlights

• 120 Strains from mother's milk were isolated and six potential strains were selected for further study.
• All the six strains were survived in stimulated bile and acidic conditions.
• Most of the Lactobacillus strains potentially prevent the growth of enteric pathogens and resistance to many antibiotics.
• All the strains showed deconjugation with sodium glycocholate and sodium taurocholate.
• Lactobacillus oris HMI68 reduced more amount of cholesterol compared with other strains.

Enrichment of amino acid-oxidizing, acetate-reducing bacteria
Makoto Ato, Masaharu Ishii, Yasuo Igarashi

Statistical analysis of sake-preparation conditions and dimethyl trisulfide formation
Kei Sasaki, Nahoko Nishibori, Muneyoshi Kanai, Atsuko Isogai, Osamu Yamada, Nami Goto-Yamamoto, Tsutomu Fujii

Functional analysis of histone deacetylase and its role in stress response, drug resistance and solid-state cultivation in Aspergillus oryzae
Moriyuki Kawauchi, Kazuhiro Iwashita

In-package atmospheric pressure cold plasma treatment of cherry tomatoes
Nrusimha Nath Misra, Kevin M. Keener, Paula Bourke, Jean-Paul Mosnier, Patrick J. Cullen

Highlights

• Cold plasma treatment of cherry tomatoes inside sealed packages.
• Emission spectroscopy of the plasma source.
• Effect on quality parameters – respiration rate, pH, firmness, and weight loss.
• Cold plasma could serve as a promising alternative to conventional processing.

Production of a biodegradable plastic-degrading enzyme from cheese whey by the phyllosphere yeast Pseudozyma antarctica GB-4(1)W
Takashi Watanabe, Yukiko Shinozaki, Ken Suzuki, Motoo Koitabashi, Shigenobu Yoshida, Yuka Sameshima-Yamashita, Hiroko Kuze Kitamoto

Biosynthesis of 1,3-propanediol from glycerol with Lactobacillus reuteri: Effect of operating variables
Jyotsna Jolly, Bernd Hitzmann, Subramanian Ramalingam, Kadathur B. Ramachandran

Highlights

• Enhanced 1,3-PD production from glycerol by co-fermentation with glucose.
• A final concentration of 65.3 g/l was obtained in repeated fed-batch fermentation.
• It is the highest reported concentration of 1,3-PD for this strain.
• The yield of 1,3-PD based on glycerol was close to the theoretical maximum value.
• The study has given insight to further improve the co-fermentation process.

Application of airlift bioreactor for the cultivation of aerobic oleaginous yeast Rhodotorula glutinis with different aeration rates
Hong-Wei Yen, Yi Xian Liu

Microcompartmentalized cell-free protein synthesis in semipermeable microcapsules composed of polyethylenimine-coated alginate
Daisuke Saeki, Shinji Sugiura, Toshiyuki Kanamori, Seigo Sato, Sosaku Ichikawa

Robust in vitro assay system for quantitative analysis of parasitic root-knot nematode infestation using Lotus japonicus
Arshana N.N. Amin, Shuhei Hayashi, Derek G. Bartlem

Observation of ovarian cancer stem cell behavior and investigation of potential mechanisms of drug resistance in three-dimensional cell culture
Junsong Chen, Jing Wang, Yunxia Zhang, Dengyu Chen, Cuiping Yang, Cai Kai, Xiaoying Wang, Fangfang Shi, Jun Dou

Highlights

• We demonstrate the HO8910 CD44+CD117+cells posses EOC CSC characteristics.
• Using 3D culture model could accurately display EOC CSC behavior in vitro.
• CD44+CD117+CSCs exhibit high chemoresistant to 5Fu, cisplatin in 3D versus 2D culture.
• EOC CSC high chemoresistance are associated with high expression of ABCG2 and MMP-2.
• 3D model improves the efficacy of screening anticancer drugs for treatment of EOC CSCs.

Efficient folding/assembly in Chinese hamster ovary cells is critical for high quality (low aggregate content) of secreted trastuzumab as well as for high production: Stepwise multivariate regression analyses
Yoichi Ishii, Junko Murakami, Kazue Sasaki, Masayoshi Tsukahara, Kaori Wakamatsu

Olfactory ensheathing cells seeded muscle-stuffed vein as nerve conduit for peripheral nerve repair: A nerve conduction study
Yogeswaran Lokanathan, Min-Hwei Ng, Shariful Hasan, Anuar Ali, Mazzre Mahmod, Ohnmar Htwe, Sharifah Ahmad Roohi, Ruszymah Bt Hj Idrus, Shalimar Abdullah, Amaramalar Selvi Naicker

Neuroprotective effect of the fermented Gumiganghwal-tang
Bo-Ra Yun, Jin Bae Weon, Jiwoo Lee, Min Rye Eom, Choong Je Ma