Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells Review Article
Hiroshi Kurosawa

Transcriptional response of Lactobacillus acidophilus L-92 after attachment to epithelial Caco-2 cells
Sae Yanagihara, Tatsuhiko Hirota, Naoyuki Yamamoto

A simple assay for 6-aminohexanoate-oligomer-hydrolase using N-(4-nitrophenyl)-6-aminohexanamide
Hisataka Taguchi, Makoto Wakamatsu, Kenji Aso, Shin Ono, Takashi Shin, Takashi Akamatsu

Gene cloning and catalytic characterization of cold-adapted lipase of Photobacterium sp. MA1-3 isolated from blood clam Original Research Article
Young Ok Kim, Vivia Khosasih, Bo-Hye Nam, Sang-Jun Lee, Antonius Suwanto, Hyung Kwoun Kim

Enhancement of 6-pentyl-α-pyrone fermentation activity in an extractive liquid-surface immobilization (Ext-LSI) system by mixing anion-exchange resin microparticles
Shinobu Oda, Sayumi Michihata, Naoki Sakamoto, Hideo Horibe, Akihiko Kono, Shinichi Ohashi

The transfer of stable 133Cs from rice to Japanese sake Original Research Article
Masaki Okuda, Midori Joyo, Masafumi Tokuoka, Tomokazu Hashiguchi, Nami Goto-Yamamoto, Hiroshi Yamaoka, Hitoshi Shimoi

Yeast ecology of vineyards within Marsala wine area (western Sicily) in two consecutive vintages and selection of autochthonous Saccharomyces cerevisiae strains Original Research Article
Luca Settanni, Ciro Sannino, Nicola Francesca, Rosa Guarcello, Giancarlo Moschetti

Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of ade1 or ade2 mutants
Marina G. Tarutina, Tatiana A. Dutova, Inna E. Yezhova, Hiroaki Nishiuchi, Sergey P. Sineoky

Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of colonies using the met30 strain
Marina G. Tarutina, Tatiana A. Dutova, Inna E. Yezhova, Hiroaki Nishiuchi, Sergey P. Sineoky

Improvement of multiple stress tolerance in yeast strain by sequential mutagenesis for enhanced bioethanol production Original Research Article
Rajni Kumari, Krishna Pramanik

Enzymatic pathway for biodegrading microcystin LR in Sphingopyxis sp. C-1 Original Research Article
Kazuya Shimizu, Hideaki Maseda, Kunihiro Okano, Takumi Kurashima, Yukio Kawauchi, Qiang Xue, Motoo Utsumi, Zhenya Zhang, Norio Sugiura

Performance of methanogenic reactors in temperature phased two-stage anaerobic digestion of swine wastewater Original Research Article
Woong Kim, Seung Gu Shin, Kyungjin Cho, Changsoo Lee, Seokhwan Hwang

Production of keratinolytic enzyme by an indigenous feather–degrading strain Bacillus cereus Wu2 Original Research Article
Wei-Hsun Lo, Jui-Rze Too, Jane-Yii Wu

Identification of an acetate-tolerant strain of Saccharomyces cerevisiae and characterization by gene expression analysis
Yutaka Haitani, Koichi Tanaka, Mami Yamamoto, Toshihide Nakamura, Akira Ando, Jun Ogawa, Jun Shima

Motility behavior of rpoS-deficient Escherichia coli analyzed by individual cell tracking Original Research Article
Yoshihiro Ojima, Kazumi Hakamada, Yosuke Nishinoue, Minh Hong Nguyen, Jun Miyake, Masahito Taya

Production of Japanese encephalitis virus-like particles using the baculovirus–insect cell system Original Research Article
Hideki Yamaji, Maiko Segawa, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi

Cell proliferation on three-dimensional chitosan–agarose–gelatin cryogel scaffolds for tissue engineering applications Original Research Article
Sumrita Bhat, Ashok Kumar

High-throughput screening method for promoter activity using bead display and a ligase ribozyme Original Research Article
Takaaki Kojima, Shoji Ohuchi, Yurie Ito, Hideo Nakano

Highlights

> In vitro high-throughput screening system for promoter activity has been developed. > Various promoter activities were analyzed by flow cytometry. > T7 promoter sequence was enriched preferentially from a pool of unrelated fragments.

A simple fed-batch method for transcription and insect cell-free translation
Yoko Sato, Keishi Aizawa, Toru Ezure, Eiji Ando, Nobuyuki Uozumi