GENETICS， MOLECULAR BIOLOGY, AND GENE ENGINEERING

Characterization of an i-type Iysozyme gene from the sea cucumber Stichopus japonicus, and enzymatic and nonenzymatic antimicrobial activities of its recombinant protein
Lina Cong, Xijian Yang, Xiuxia Wang, Mikiro Tada, Meiling Lu, Heng Liu, and Beiwei Zhu

Characterization of a novel rat gene RTAP2a, screened by cross-reactive SEREX, restrictedly expressed in testis
Hanshuo Yang, Chunting Wang, Rui Wang, Hongxin Deng, Zhenyu Ding, Jinliang Yang, You Lu, Jiong Li, Peng Zhang, Yongqiu Mao, Bing Kan, Lin Wei, Feng Peng, and Yuquan Wei

One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene
Kazuhiro Machida, Yasuhide Aritoku, and Toshio Tsuchida

Catalytic properties of a lipase from Photobacterium lipolyticum for biodiesel production containing a high methanol concentration
Kyung Seok Yang, Jung-Hoon Sohn, and Hyung Kwoun Kim

Properties and substrate specificities of Proteolytic enzymes from the edible basidiomycete Grifola frondosa
Toshikazu Nishiwaki, Satoshi Asano, and Takuji Ohyama

Isolation and characterization of a multienzyme complex (cellulosome) of the Paenibacillus curdlanolyticus B-6 grown on Avicel under aerobic conditions
Rattiya Waeonukul, Khin Lay Kyu, Kazuo Sakka, and　Khanok Ratanakhanokchai

Purification and identification of proteolytic enzymes from Aspergillus oryzae capable of producing the antihypertensive peptide Ile-Pro-Pro
Takanobu Gotou, Tadashi Shinoda, Seiichi Mizuno, and Naoyuki Yamamoto

Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca
Takanori Ambo, Motoyoshi Noike, Hirofumi Kurokawa, and Tanetoshi Koyama

Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6’:2’,6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system
Nobuchika Takesue，Teruo Sone, Michiko Tanaka, Fusao Tomita，and Kozo Asano

Biofilm formation by Escherichia coli in hypertonic sucrose media
Taketo Kawarai, Soichi Furukawa, Naoki Narisawa, Chisato Hagiwara, Hirokazu Ogihara, and Makari Yamasaki

Disruption of ubiquitin-related genes in laboratory yeast strains enhances ethanol production during sake brewing
Hong Wu, Tomoko Watanabe, Yoshio Araki, Hiroshi Kitagaki, Takeshi Akao, Hiroshi Takagi, and Hitoshi Shimoi

Enhanced treatment efficiency of an anaerobic sequencing batch reactor (ASBR) for cassava stillage with high solids content
Gang Luo, Li Xie, and Qi Zhou

Novel two-dimensional DNA gel electrophoresis mapping for characterizing complex bacterial communities in environmental samples
Guo-hua Liu, Toshiyuki Harada, Takashi Amemiya, and Kiminori Itoh

Caspase inhibitors increase the rate of recovery of neural stem/progenitor cells from post-mortem rat brains stored at room temperature
Atsuko Hasegawa, Chikako Yamada, Miho Tani, Shun-ichiro Hirano, Yasuhito Tokumoto, and Jun Miyake

A rapid and efficient method for directed screening of lipase-producing Burkholderia cepacia complex strains with organic solvent tolerance from rhizosphere
Zhengyu Shu, Ruifeng Lin, Huan Jiang, Yanfeng Zhang, Mingzi Wang, and Jianzhong Huang

Affinity capillary electrophoresis with magnetic beads for multiplex quantitative analysis of bacterial 16S rRNA
Ken Adachi, Masahiro Yamaguchi, Makoto Nakashige, Takahiro Kanagawa, Masaki Torimura, Satoshi Tsuneda, Yuji Sekiguchi, and Naohiro Noda

Preparation of long sticky ends for universal ligation-independent cloning: Sequential T4 DNA polymerase treatments
Akira Tachibana, Kazuo Tohiguchi, Takayuki Ueno, Yuichi Setogawa, Ayako Harada, and Toshizumi Tanabe