GENETICS， MOLECULAR BIOLOGY, AND GENE ENGINEERING

Deletion analysis of the promoter of Aspergillus oryzae gene encoding heat shock protein 30
Mayumi Matsushita, Sawaki Tada, Satoshi Suzuki, Ken-Ichi Kusumoto, and Yutaka Kashiwagi

Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80
Yoshito Suda, Tomoaki Yoshikawa, Yuko Okuda，Mei Tsunemoto, Satoshi Tanaka, Kazunori Ikeda, Hitoshi Miyasaka, Masanori Watanabe, Ken Sasaki, Kazuo Harada, Takeshi Bamba, and Kazumasa Hirata

ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY

Stability of hen egg white lysozyme during denaturation is enhanced by pretreatment with supercritical carbon dioxide
Steven S.-S. Wang, Hsuan-Shen Chao, Hsuan-Liang Liu, and Hwai-Shen Liu

Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5
Amir Jalal, Naeem Rashid, Nouman Rasool, and Muhammad Akhtar

Using response surface methodology to optimize process parameters and cross-linking agents for production of combined-cross-linked bovine serum albumin gels
Chee-Yuen Gan, Abbas F. M. Alkarkhi, and Azhar Mat Easa

Purification and characterization of an eggshell membrane decomposing protease from Pseudomonas aeruginosa strain ME-4
Minyi Cheng, Shinji Takenaka, Shunsuke Aoki, Shuichiro Murakami, and Kenji Aoki

MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY

Characterization of a spontaneous flocculation mutant derived from Candida glabrata : A useful strain for　bioethanol production
Itsuki Watanabe, Toshihide Nakamura，and Jun Shima

BREWING AND FOOD TECHNOLOGY

QTL mapping of sake brewing characteristics of yeast
Taku Katou, Masahiro Namise, Hiroshi Kitagaki， Takeshi Akao， and Hitoshi Shimoi

ENVIRONMENTAL BIOTECHNOLOGY

Intergeneric coaggregation of non-flocculating Acinetobacter spp. isolates with other sludge-constituting bacteria
Kimchhayarasy Phuong, Kazuo Kakii， and Toshiyuki Nikata

Symbiotic effects of a lipase-secreting bacterium, Burkholderia arboris SL1B1，and a glycerol-assimilating yeast， Candida cylindracea SL1B2， on triacylglycerol degradation
Hiroshi Matsuoka， Atsuto Miura，and Katsutoshi Hori

Dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum
Sung-Jin Jo， Chean Ring Leong， Ken'ichiro Matsumoto， and Seiichi Taguchi

BIOCHEMICAL ENGINEERING

Culture scale-up studies as seen from the viewpoint of oxygen supply and dissolved carbon dioxide stripping
Naoki Matsunaga, Kenjiro Kano，Yasuyuki Maki， and Toshiaki Dobashi

Estimation of dissolved carbon dioxide stripping in a large bioreactor using model medium
Naoki Matsunaga, Kenjiro Kano，Yasuyuki Maki， and Toshiaki Dobashi

Application of electrodialysis to glycerate recovery from a glycerol containing model solution and culture broth
Hiroshi Habe， Tokuma Fukuoka, Dai Kitamoto， and Keiji Sakaki

PLANT BIOTECHNOLOGY

Oenothera laciniata inhibits lipopolysaccharide induced production of nitric oxide， prostaglandin E₂，and　proinflammatory cytokines in RAW264.7 macrophages
Weon-Jong Yoon， Young Min Ham， Byoung-Sam Yoo， Ji-Young Moon, Jaesook Koh， and Chang-Gu Hyun

Improved procedure for nucleus extraction for DNA measurements by flow cytometry of red beet (Beta vulgaris L.) hairy roots
Vasil Georgiev, Jost Weber， Thomas Bley, and Atanas Pavlov

CELL AND TISSUE ENGINEERING

Development of cystic embryoid bodies with visceral yolk-sac-like structures from mouse embryonic stem　cells using low-adherence 96-well Plate
Emiko Yasuda, Yuji Seki, Takatoshi Higuchi, Fumio Nakashima，　Tomozumi Noda, and Hiroshi Kurosawa

Differentiation of dopaminergic neurons from human embryonic stem cells: Modulation of differentiation by FGF-20
Hideaki Shimda, Naoko Yoshimura， Akiko Tsuji, and Takahiro Kunisada

MEDICAL BIOTECHNOLOGY

Sentinel lymph node mapping using near-infrared fluorescent methylene blue
Maoquan Chu， and　Yinghan Wan

Cloning and expression of a novel insulin-releasing peptide, brevinin-2GU from Escherichia coli
Qing-feng Zhou， Ming-yue Li， and Cheng-wei Li

METHODS

Comparison of dry- and wet-based fine bead homogenizations to extract DNA from fungal spores
Naomichi Yamamoto， Yasunari Matsuzaka, Minoru Kimura, Hideaki Matsuki, and Yukio Yanagisawa

Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids
Fumito　Maruyama, Takashi Nozawa， Chihiro Aikawa， Atsuo Sakurai， and Ichiro Nakagawa

MISCELLANEOUS

Effect of Transforming Growth Factor-β1 on Morphological Characteristics Relating to Migration and Differentiation of Rabbit Chondrocytes Cultured in Collagen Gels
Ali Baradar Khoshfetrat, Masahiro Kino-oka， Yasunori Takezawa, Yasuaki Sato，Takeyuki Yamamoto， Katsura Sugawara， and Masahito Taya