GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING

Ethanol stress stimulates the Ca²⁺ -mediated calcineurin/Crz1 pathway in Saccharomyces cerevisiae
Yoshio Araki， Hong Wu， Hiroshi Kitagaki， Takeshi Akao， Hiroshi Takagi， and Hitoshi Shimoi

Identification of mutation sites on ω3 desaturase genes from Mortierella alpina 1S-4 mutants
Eiji Sakuradani，Takahiro Abe， and Sakayu Shimizu

ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY

Enzymatic characteristics of a Ser/Thr protein kinase， SpkA， from Myxococcus xanthus
Yoshio Kimura，Aoi Kakemizu， Yuuko Matsubara， and Kaoru Takegawa

Production of L-malic acid with fixation of HCO₃ by malic enzyme-catalyzed reaction based on regeneration of coenzyme on electrode modified by layer-by-layer self-assembly method
Haitao Zheng, Yoko Ohno， Toshihiko Nakamori， and Shin-ichiro Suye

Gene cloning and enzymatic properties of hyperthermostable β-glycosidase from Thermus thermophilus HJ6
Na-Yeon Gu， Jung-Lim Kim， Hyun-Jung Kim， Dong-Ju You， Han-Woo Kim， and Sung-Jong Jeon

Gene cloning and characterization of arylamine N-acetyltransferase from Bacillus cereus strain 10-L-2
Shinji Takenaka, Minyi Cheng, Mulyono，Atsushi Koshiya， Shuichiro Murakami， and Kenji Aoki

Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus
Jae-Won Lee， Jun-Yeong Park, Mi Kwon, and In-Gyu Choi

Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium，Bacillus subtilis WU-S2B
Shusuke Takahashi, Toshiki Furuya, Yoshitaka Ishii, Kuniki Kino, and Kohtaro Kirimura

BREWING AND FOOD TECHNOLOGY

Comparative study of antioxidant activity and antiproliferative effect of hot water and ethanol extracts from the mushroom Inonotus obliquus
Honghai Hu, Zhenya Zhang, Zhongfang Lei, Yingnan Yang, and Norio Sugiura

ENVIRONMENTAL BIOTECHNOLOGY

Treatment of high-strength corn steep liquor using cultivated Polyvinyl alcohol gel beads in an anaerobic fluidized-bed reactor
Wenjie Zhang, Qinglin Xie， Joseph D. Rouse， Sen Qiao， and Kenji Furukawa

Thermophilic anaerobic co-digestion of garbage, screened swine and dairy cattle manure
Kai Liu, Yue-Qin Tang, Toru Matsui， Shigeru Morimura, Xiao-Lei Wu， and Kenji Kida

Enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria from sediment of sea-based waste disposal site
Yasunori Kawagoshi， Yasuhiro Nakamura， Hirotaka Kawashima， Koichiro Fujisaki， Aya Fujimoto， and Kenji Furukawa

Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora
Hiroshi Yokoyama, Hideyuki Ohmori, Miyoko Waki, Akifumi Ogino， and Yasuo Tanaka

BIOCHEMICAL ENGINEERING

Human single-chain antibody expression in the hemolymph and fat body of silkworm larvae and pupae using BmNPV bacmids
Motoki Ishikiriyama, Takuya Nishina, Tatsuya Kato, Hiroshi Ueda, and Enoch Y. Park

Efficient production of single-chain Fv antibody possessing rare codon linkers in fed-batch fermentation
Yoichi Kumada， Yoshinobu Sakan， Hideyuki Kajihara， Mana Kihara， Yasufumi Kikuchi， Hideki Yamaji， Gi Hun Seong， and Shigeo Katoh

PLANT BIOTECHNOLOGY

Higher-level accumulation of foreign gene products in transgenic rice seeds by the callus-specific selection system
Yuhya Wakasa, Kenjirou Ozawa, and Fumio Takaiwa

BIOINFORMATICS

Maximum entropy decomposition of flux distribution at steady state to elementary modes
Quanyu Zhao and Hiroyuki Kurata

METHODS

Classification of pollen species using autofluorescence image analysis
Kotaro Mitsumoto， Katsumi Yabusaki，and Hideki Aoyagi

Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with　a mutation primer
Yu Kato， Noriaki Arakawa, Yusuke Masuishi， Hiroshi Kawasaki， and Hisashi Hirano