■　REVIEW

Developments in Biotechnological Production of Sweet Proteins.
Tetsuya Masuda and Naofumi Kitabatake

Establishment of Ovarian Reconstruction System in Culture for Functional Genomic Analysis.
Chang-Eun Park, Dongryul Lee, Kyeoung-Hwa Kim, and Kyung-Ah Lee.

Identification of Genes from Aspergillus oryzae That Are Preferentially Expressed in Membrane-Surface Liquid Culture.
Bin Feng, Masakazu Morita, Hiroyuki Imanaka, Koreyoshi Imamura, and Kazuhiro Nakanishi

Novel Substrate Specificity of D-Arabinose Isomerase from Klebsiella pneumoniae and Its Application to Production of D-Altrose from D-Psicose.
Buetusiwa Thomas Menavuvu, Wayoon Poonperm, Kosei Takeda, Kenji Morimoto, Tom Birger Granstrom, Goro Takada, and Ken Izumori

Determination of Enzymes from Colletotrichum sp. AHU9748 Essential for Lepidimoide Production from Okra Polysaccharide.
Chayaporn Saranpuetti, Michiko Tanaka, Teruo Sone, Kozo Asano, and Fusao Tomita 　

MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY

Lactate and Ethanol Productions by Rhizopus oryzae ATCC 9363 and Activities of Related Pyruvate Branch Point Enzymes.
Ali Oguz Buyukkileci, Haluk Hamamci, and Meral Yucel　

ENVIRONMENTAL BIOTECHNOLOGY

Involvement of Radical Species in Inactivation of Vibrio parahaemolyticus in Saline Solutions by Direct-Current Electric Treatment.
Hiromi Urano, Hiroshi Ishikawa, and Satoshi Fukuzaki　

BIOCHEMICAL ENGINEERING

Cultivation of Recombinant Chinese Hamster Ovary Cells Grown as Suspended Aggregates in Stirred Vessels.
Yi Han, Xing-Mao Liu, Hong Liu, Shi-Chong Li, Ben-Chuan Wu, Ling-Ling Ye, Qu-Wei Wang, and Zhao-Lie Chen

Effect of Flashing Light from Blue Light Emitting Diodes on Cell Growth and Astaxanthin Production of Haematococcus pluvialis.
Tomohisa Katsuda, Kazumichi Shimahara, Hironori Shiraishi, Keisuke Yamagami, Reza Ranjbar, and Shigeo Katoh

Glycosylation Variant Analysis of Recombinant Human Tissue Plasminogen Activator Produced in Urea-Cycle-Enzyme- Expressing Chinese Hamster Ovary (CHO) Cell Line.
Hyoung Jin Kim and Hong-Jin Kim

Pretreatment of Polyamide Monofilament with Hydrochloric Acid Improves Sensitivity of Three-Dimensional Microarray, Bio-Strand.
Yuriko Tojo, Reishi Syou, Mamiko Yoshida, Junko Momose, Harumi Ginya, Masaaki Takahashi, Hideji Tajima, and Masafumi Yohda

PLANT BIOTECHNOLOGY

ZMVHA-B1, the Gene for Subunit B of Vacuolar H⁺-ATPase from the Eelgrass Zostera marina L. Is Able to Replace vma2 in a Yeast Null Mutant.
Abbas Alemzadeh, Makoto Fujie, Shoji Usami, Tsukasa Yoshizaki, Keiji Oyama, Toyoki Kawabata, and Takashi Yamada

CELL AND TISSUE ENGINEERING

Biological Responses of Ligament Fibroblasts and Gene Expression Profiling on Micropatterned Silicone Substrates Subjected to Mechanical Stimuli.
Su A Park, In Ae Kim, Yong Jae Lee, Ji Won Shin, Chong-Rak Kim, Jeong Koo Kim, Young-Il Yang, and Jung-Woog Shin

Ex Vivo Expansion of Human Cord Blood Hematopoietic Progenitor Cells Using Glutaraldehyde-Fixed Human Bone Marrow Stromal Cells.
Yoshihiro Ito, Hirokazu Hasauda, Takashi Kitajima, and Toru Kiyono 　

MEDICAL BIOTECHNOLOGY

Modified Keratin Sponge: Binding of Bone Morphogenetic Protein-2 and Osteoblast Differentiation.
Akira Tachibana, Yuji Nishikawa, Masaaki Nishino, Sumika Kaneko, Toshizumi Tanabe, and Kiyoshi Yamauchi

BIOINFORMATICS

Precise Metabolic Flux Analysis of Coryneform Bacteria by Gas Chromatography-Mass Spectrometry and Verification by Nuclear Magnetic Resonance.
Tomokazu Shirai, Kunihiro Matsuzaki, Masanori Kuzumoto, Keisuke Nagahisa, Chikara Furusawa, Suteaki Shioya, and Hiroshi Shimizu

METHODS

Novel In Vitro Screening System for Monoclonal Antibodies Using Hypermutating Chicken B Cell Library.
Kagefumi Todo, Kenji Miyake, Masaki Magari, Naoki Kanayama, and Hitoshi Ohmori

16S rRNA Gene-Based Analysis of Microbial Community by Whole-Genome Amplification and Minigel-Single-Strand Conformation Polymorphism Technique.
Michiei Oto, Wataru Suda, and Hirofumi Shinoyama